J Korean Neurol Assoc.
1997 Jun;15(3):455-462.
The expression of phospholipase C and Siah gene product during murine fetal development by immunohistochemistry
- Affiliations
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- 1Department of Neurology, College of Medicine, Ewha Womans University.
- 2Department of Pathology, College of Medicine, University of Ulsan.
- 3Department of life science, Pohang Institute of Science and Technology.
Abstract
- Recently identified second messenger molecules of signal transmission were derived from inositol phospholipid and several phospholipase C(PLC) isoenzyme were reconized. But the role of PLC in embryonal organ development, differentiation and growth is not described, yet. And, Siah gene of mouse is expressed at a higher level in a restricted number of sites during development. So, it is suggested that the Siah gene plays a significant role in development of vertebrates.Therefore, this study was aimed to investigate the role of PLC isoenzymes and Siah gene during murine fetal development by use of immunehistochemistry. The results are as follow: 1. PLC-beta1 and PLC-gammal are expressed apparently at early fetal stage, PLC-betal is observed focally, whereas PLC-gamma1 is diffusely expressed. 2. PLC-delta1 is not expressed during early and mid murine fetal age but strongly expressed in placental tissue. 3. PLC-beta1 is observed mainly in neural tissues including brain at mid fetal stage. PLC-gamma1 is expressed in entire organs, especially in liver tissue, and then their expression is reduced after mid stage. 4. Siah gene product is significantly expressed in olfactory epithelium, retina, neuron of brain and choroid plexus from mid fetal stage to late gestation. These results suggest that PLC-betal play a important role in central neural development and PLC-gamma1 in entire orgaans, especially in liver development. The strong expression of PLC-deltal in placenta suggest its involvement in early development, indirectly. Siah gene product expressed at a higher levels in olfactory epithelium, retina, neuron and choroid plexus, so Siah gene may play a important role in these organ developments.
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