J Clin Pathol Qual Control.
1999 Jun;21(1):229-235.
Development of the Home-Made Panel Tray for Panel Reactive Antibody Test
- Affiliations
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- 1Department of Clinical Pathology, University of Ulsan College of Medicine, Ulsan University Hospital, Ulsan, Korea. suncp@uuh.ulsan.kr
- 2Department of Internal Medicine, University of Ulsan College of Medicine, Ulsan University Hospital, Ulsan, Korea.
- 3Department of General Surgery, University of Ulsan College of Medicine, Ulsan University Hospital, Ulsan, Korea.
- 4Department of Orthopedic Surgery, University of Ulsan College of Medicine, Ulsan University Hospital, Ulsan, Korea.
- 5Department of Urology, University of Ulsan College of Medicine, Ulsan University Hospital, Ulsan, Korea.
- 6Department of Clinical Pathology, Pusan National University Medical School, Pusan, Korea.
Abstract
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BACKGROUND: The panel reactive antibody (PRA) test is critical for predicting the probability of cadaver transplantation to possible organ recipients. In Korea, only a few institutions are performing the PRA test and there are some limitations in the use of commercial PRA test kits.
METHODS
Two sets of home-made lymphocyte panel trays were made for the PRA test, using the lymphocytes of hospital workers. PRA tests were carried out for 33 patients with chronic renal failure using the two sets of PRA trays.
RESULTS
The two panels were containing most of the HLA class I antigens with more than one percent of frequency in Koreans. Of 33 patients with chronic renal failure, 16 showed positive PRA test in at least one lymphocyte panel. In two patients, the HLA cass I antibodies were identified by PRA tests: anti-A2 and anti-A24 antibodies in one patient, and anti-B54 antibody in the other.
CONCLUSION
The home-made lymphocyte panel can be used effectively for PRA tests of possible organ recipients.
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