Korean Lepr Bull.  2010 Dec;43(1):3-13.

The usefulness of multiple displacement amplification in the molecular studies in Hansen's disease

• Kim JP

Abstract

Appreciable amounts of high-quality DNA are frequently required for use in molecular diagnostic and epidemiology studies. The limited availability of DNA can be a critical obstacle to meeting research and clinical needs. And molecular methods have not been routinely applied to the obligate intracellular organism Mycobacterium leprae because of the difficulty of obtaining a genomic DNA template from clinical material. The MDA (multiple displacement amplification) reaction is increasingly the method of choice for many applications because of its extensive coverage of the genome, the generation of extremely long DNA products compared with older whole genome amplification methods and the high DNA yields, even from exceedingly low amounts of starting material. In the study, the author evaluated the usefulness of this method for the molecular studies in Hansen's disease.

Keyword

MDA (multiple displacement amplification); Hansen's disease

MeSH Terms

Displacement (Psychology)
DNA
Genome
Leprosy
Mycobacterium leprae
Pathology, Molecular
DNA