Obstet Gynecol Sci.  2014 Sep;57(5):373-378. 10.5468/ogs.2014.57.5.373.

Dose-dependent embryotrophic effect of recombinant granulocyte-macrophage colony-stimulating factor and brain-derived neurotrophic factor in culture medium for mouse preimplantation embryo

• Kim JH
• Lee HJ
• Yu EJ
• Jee BC
• Suh CS
• Kim SH

• KMID: 2051718
• DOI: http://doi.org/10.5468/ogs.2014.57.5.373

Abstract

OBJECTIVE
To evaluate the dose effect of recombinant mouse granulocyte-macrophage colony-stimulating factor (rmGM-CSF) or brain-derived neurotrophic factor (BDNF) in culture medium on the development of in vitro fertilized mouse embryos.
METHODS
Mature oocytes were retrieved from superovulated female BDF1 mice and inseminated by sperm from male BDF1 mice. On day 1, two-cell stage embryos were divided and cultured until day 5 in the embryo maintenance medium supplemented with 0, 1, 2, 5, or 10 ng/mL of rmGM-CSF or supplemented with 0, 5, 10, or 20 ng/mL of BDNF. Blastocyst formation rate and their cell numbers were assessed.
RESULTS
The blastocyst formation rate and the total cell count in blastocyst was similar in all the rmGM-CSF treatment groups when compared with the control. However, the blastocyst formation rate and the total cell count was significantly higher in the group supplemented with 10 ng/mL of BDNF compared with the control (63.9%, 45.8+/-11.5 vs. 52.3%, 38.0+/-6.8; P<0.05, respectively).
CONCLUSION
Supplementation of 10 ng/mL of BDNF enhanced the developmental potential of mouse preimplantation embryos, but supplementation of rmGM-CSF did not.

Keyword

Brain-derived neurotrophic factor; Culture medium; Embryotrophic effects; Granulocyte-macrophage colony-stimulating factor

MeSH Terms

Animals
Blastocyst*
Brain-Derived Neurotrophic Factor*
Cell Count
Embryonic Structures
Female
Granulocyte-Macrophage Colony-Stimulating Factor*
Humans
Male
Mice*
Oocytes
Spermatozoa
Brain-Derived Neurotrophic Factor
Granulocyte-Macrophage Colony-Stimulating Factor

Reference

1. Gardner DK, Lane M, Calderon I, Leeton J. Environment of the preimplantation human embryo in vivo: metabolite analysis of oviduct and uterine fluids and metabolism of cumulus cells. Fertil Steril. 1996; 65:349–353.

2. Gardner DK, Vella P, Lane M, Wagley L, Schlenker T, Schoolcraft WB. Culture and transfer of human blastocysts increases implantation rates and reduces the need for multiple embryo transfers. Fertil Steril. 1998; 69:84–88.

3. Richter KS. The importance of growth factors for preimplantation embryo development and in-vitro culture. Curr Opin Obstet Gynecol. 2008; 20:292–304.

4. Aflalo ED, Sod-Moriah UA, Potashnik G, Har-Vardi I. EGF increases expression and activity of PAs in preimplantation rat embryos and their implantation rate. Reprod Biol Endocrinol. 2007; 5:4.

5. Papayannis M, Eyheremendy V, Sanjurjo C, Blaquier J, Raffo FG. Effect of granulocyte-macrophage colony stimulating factor on growth, resistance to freezing and thawing and re-expansion of murine blastocysts. Reprod Biomed Online. 2007; 14:96–101.

6. Zhou P, Liu DJ, Cang M, Ma YZ, Yang DS, Li HJ, et al. TGFalpha and EGFR in ovine preimplantation embryos and effects on development. Anim Reprod Sci. 2008; 104:370–381.

7. Ruef C, Coleman DL. Granulocyte-macrophage colony-stimulating factor: pleiotropic cytokine with potential clinical usefulness. Rev Infect Dis. 1990; 12:41–62.

8. Caceres-Cortes J, Rajotte D, Dumouchel J, Haddad P, Hoang T. Product of the steel locus suppresses apoptosis in hemopoietic cells: comparison with pathways activated by granulocyte macrophage colony-stimulating factor. J Biol Chem. 1994; 269:12084–12091.

9. Rajotte D, Haddad P, Haman A, Cragoe EJ Jr, Hoang T. Role of protein kinase C and the Na+/H+ antiporter in suppression of apoptosis by granulocyte macrophage colony-stimulating factor and interleukin-3. J Biol Chem. 1992; 267:9980–9987.

10. Williams GT, Smith CA, Spooncer E, Dexter TM, Taylor DR. Haemopoietic colony stimulating factors promote cell survival by suppressing apoptosis. Nature. 1990; 343:76–79.

11. Giacomini G, Tabibzadeh SS, Satyaswaroop PG, Bonsi L, Vitale L, Bagnara GP, et al. Epithelial cells are the major source of biologically active granulocyte macrophage colony-stimulating factor in human endometrium. Hum Reprod. 1995; 10:3259–3263.

12. Zhao Y, Chegini N. Human fallopian tube expresses granulocyte-macrophage colony stimulating factor (GM-CSF) and GM-CSF alpha and beta receptors and contain immunoreactive GM-CSF protein. J Clin Endocrinol Metab. 1994; 79:662–665.

13. Robertson SA, Sjoblom C, Jasper MJ, Norman RJ, Seamark RF. Granulocyte-macrophage colony-stimulating factor promotes glucose transport and blastomere viability in murine preimplantation embryos. Biol Reprod. 2001; 64:1206–1215.

14. Sjoblom C, Wikland M, Robertson SA. Granulocyte-macrophage colony-stimulating factor promotes human blastocyst development in vitro. Hum Reprod. 1999; 14:3069–3076.

15. Barbacid M. The Trk family of neurotrophin receptors. J Neurobiol. 1994; 25:1386–1403.

16. Kafitz KW, Rose CR, Thoenen H, Konnerth A. Neurotrophin-evoked rapid excitation through TrkB receptors. Nature. 1999; 401:918–921.

17. Seifer DB, Feng B, Shelden RM, Chen S, Dreyfus CF. Brain-derived neurotrophic factor: a novel human ovarian follicular protein. J Clin Endocrinol Metab. 2002; 87:655–659.

18. Dissen GA, Hirshfield AN, Malamed S, Ojeda SR. Expression of neurotrophins and their receptors in the mammalian ovary is developmentally regulated: changes at the time of folliculogenesis. Endocrinology. 1995; 136:4681–4692.

19. Anderson RA, Bayne RA, Gardner J, De Sousa PA. Brain-derived neurotrophic factor is a regulator of human oocyte maturation and early embryo development. Fertil Steril. 2010; 93:1394–1406.

20. Kawamura K, Kawamura N, Mulders SM, Sollewijn Gelpke MD, Hsueh AJ. Ovarian brain-derived neurotrophic factor (BDNF) promotes the development of oocytes into preimplantation embryos. Proc Natl Acad Sci U S A. 2005; 102:9206–9211.

21. Kawamura K, Kawamura N, Fukuda J, Kumagai J, Hsueh AJ, Tanaka T. Regulation of preimplantation embryo development by brain-derived neurotrophic factor. Dev Biol. 2007; 311:147–158.

22. Elaimi A, Gardner K, Kistnareddy K, Harper J. The effect of GM-CSF on development and aneuploidy in murine blastocysts. Hum Reprod. 2012; 27:1590–1595.

23. Kwak SS, Jeung SH, Biswas D, Jeon YB, Hyun SH. Effects of porcine granulocyte-macrophage colony-stimulating factor on porcine in vitro-fertilized embryos. Theriogenology. 2012; 77:1186–1197.

24. Jee BC, Jo JW, Lee JR, Suh CS, Kim SH, Moon SY. Effect of trichostatin A on fertilization and embryo development during extended culture of mouse oocyte. Zygote. 2012; 20:27–32.

25. Behr B, Mooney S, Wen Y, Polan ML, Wang H. Preliminary experience with low concentration of granulocyte-macrophage colony-stimulating factor: a potential regulator in preimplantation mouse embryo development and apoptosis. J Assist Reprod Genet. 2005; 22:25–32.

26. Desai N, Kattal N, AbdelHafez FF, Szeptycki-Lawson J, Goldfarb J. Granulocyte-macrophage colony stimulating factor (GM-CSF) and co-culture can affect post-thaw development and apoptosis in cryopreserved embryos. J Assist Reprod Genet. 2007; 24:215–222.

27. Sjoblom C, Roberts CT, Wikland M, Robertson SA. Granulocyte-macrophage colony-stimulating factor alleviates adverse consequences of embryo culture on fetal growth trajectory and placental morphogenesis. Endocrinology. 2005; 146:2142–2153.

28. Karagenc L, Lane M, Gardner DK. Granulocyte-macrophage colony-stimulating factor stimulates mouse blastocyst inner cell mass development only when media lack human serum albumin. Reprod Biomed Online. 2005; 10:511–518.

29. Sjoblom C, Wikland M, Robertson SA. Granulocyte-macrophage colony-stimulating factor (GM-CSF) acts independently of the beta common subunit of the GM-CSF receptor to prevent inner cell mass apoptosis in human embryos. Biol Reprod. 2002; 67:1817–1823.

30. Ziebe S, Loft A, Povlsen BB, Erb K, Agerholm I, Aasted M, et al. A randomized clinical trial to evaluate the effect of granulocyte-macrophage colony-stimulating factor (GM-CSF) in embryo culture medium for in vitro fertilization. Fertil Steril. 2013; 99:1600–1609.

31. Agerholm I, Loft A, Hald F, Lemmen JG, Munding B, Sorensen PD, et al. Culture of human oocytes with granulocyte-macrophage colony-stimulating factor has no effect on embryonic chromosomal constitution. Reprod Biomed Online. 2010; 20:477–484.