J Korean Rheum Assoc.
2003 Sep;10(3):261-269.
Chondrocyte Viability in the Three Dimensional Culture Using Natural Alginate
- Affiliations
-
- 1Department of Laboratory Medicine, Catholic University of Daegu School of Medicine. sgkim@cataegu.ac.kr
- 2Department of Internal Medicine, Catholic University of Daegu School of Medicine.
- 3Department of Radiology, Catholic University of Daegu School of Medicine.
- 4Department of Biomedical Engineering, Catholic University of Daegu School of Medicine.
- 5Department of Biostatistics, Catholic University of Daegu School of Medicine.
- 6Department of Biology, Catholic University of Daegu.
Abstract
OBJECTIVE
Articular cartilage has a highly limited capacity to repair because of lack of blood supply. There have been no effective modality to regenerate the articular cartilage and prevent degenerative changes. It is necessary to proliferate the cells in vitro, however the cells lose their phenotype during in vitro monolayer culture. Although it is not enough to increase the number of the cells in the three dimensional culture, it is a effective way to maintain their original phenotype expression. Alginate has been used as a good source of scaffold in chondrocyte three dimensional culture. The objective of this study was to find the most favorable scaffold for chondrocyte viability among various alginate extracted from natural source in chondrocyte three dimensional culture.
METHODS
The alginate extracted from brown seaweed, Undaria pinnatifida and sea tangle, Laminaria japonica inhabitating near Korean sea and commercially available alginate were used. Chondrocytes isolated from adult pig were used. Three kinds of chondrocyte-alginate bead were made and incubated for forty-four days. Cellular viability and glycosaminoglycan (GAG) content were measured and compared. SPSS Version 10.0 was used for data analysis.
RESULTS
Viability of chondrocyte and GAG content were increased as a function of time. Alginate from brown seaweed, U. pinnatifida appeared more favorable to maintain chondrocyte viability than others. The total GAG content was similar among three kinds of alginate
CONCLUSION
Alginate extracted from natural see weed, especially brown seaweed, may be a good source to maintain chondrocyte viability in three dimensional culture.
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