J Periodontal Implant Sci.  2014 Jun;44(3):126-133. 10.5051/jpis.2014.44.3.126.

Evaluation of interleukin-1beta and 8 in gutka chewers with periodontitis among a rural Indian population

Affiliations
  • 1Department of Clinical Dentistry, International Medical University School of Dentistry, Kuala Lumpur, Malaysia. Shaju_Jacob@imu.edu.my
  • 2Department of Periodontology, Vananchal Dental College and Hospital, Garhwa, India.
  • 3Department of Periodontology, Chhattisgarh Dental College and Research Institute, Rajnandgaon, India.

Abstract

PURPOSE
Smokeless tobacco-based oral-use products like gutka are popular in India. Gutka usage leads to increased periodontal destruction and inflammation; however, the relevant mechanism remains unknown. This study aimed to elucidate the role of gutka in periodontitis by examining its effect on the levels of interleukin (IL) 1beta and IL-8 from the gingival crevicular fluid (GCF).
METHODS
A total of 45 patients were enrolled in this study. Thirty patients with periodontitis (15 gutka chewers [GCP] and 15 nongutka chewers [NGC]) and 15 periodontally healthy controls (HC) were selected. The full-mouth plaque index (PI), gingival index (GI), probing depth (PD), clinical attachment level (CAL), and recession (RC) were recorded. The IL-1beta and IL-8 levels in the GCF of all subjects were assessed through an enzyme-linked immunosorbent assay (Quantikine).
RESULTS
The IL-1beta and IL-8 levels were not significantly higher in the GCP group (IL-1beta, 369.01+/-273.44 microL; IL-8, 205.97+/-196.78 microL) as compared to those in the NGC group (IL-1beta, 195.57+/-96.85 microL; IL-8, 178.61+/-149.35 microL). More gingival RC and loss of attachment was seen among the GCP group (RC: 2.02+/-0.31, P=0.013; CAL: 4.60+/-0.56, P<0.001) than among the NGC group (RC, 1.21+/-1.15; CAL, 3.70+/-0.32); however, PD was deeper among the NGC subjects (P=0.002). PI and GI were significantly higher for the periodontitis group (P<0.001) when compared to the HC, but there was no difference among gutka chewers and non-chewers (P=0.22 and P=0.89). A positive correlation was found between the IL-8 levels and the duration of gutka chewing (r=-0.64, P<0.01).
CONCLUSIONS
Gutka chewing leads to increased gingival RC and clinical loss of attachment. There was no effect seen in the proinflammatory cytokine levels in the GCF of gutka users.

Keyword

Cytokine; Interleukin-1beta; Interleukin-8; Periodontitis; Smokeless tobacco

MeSH Terms

Enzyme-Linked Immunosorbent Assay
Gingival Crevicular Fluid
Humans
India
Inflammation
Interleukin-1beta*
Interleukin-8
Interleukins
Lobeline
Mastication
Periodontal Index
Periodontitis*
Tobacco, Smokeless
Interleukin-1beta
Interleukin-8
Interleukins
Lobeline

Reference

1. World report on the global tobacco epidemic, 2008. The MPOWER package [Internet]. Geneva: World Health Organization;c2008. cited 2013 Nov 15. Available from: http://www.who.int/tobacco/mpower/mpower_report_full_2008.pdf.
2. Grossi SG, Zambon JJ, Ho AW, Koch G, Dunford RG, Machtei EE, et al. Assessment of risk for periodontal disease. I. Risk indicators for attachment loss. J Periodontol. 1994; 65:260–267.
Article
3. Tymkiw KD, Thunell DH, Johnson GK, Joly S, Burnell KK, Cavanaugh JE, et al. Influence of smoking on gingival crevicular fluid cytokines in severe chronic periodontitis. J Clin Periodontol. 2011; 38:219–228.
Article
4. Bergström J. Periodontitis and smoking: an evidence-based appraisal. J Evid Based Dent Pract. 2006; 6:33–41.
Article
5. Sujatha D, Hebbar PB, Pai A. Prevalence and correlation of oral lesions among tobacco smokers, tobacco chewers, areca nut and alcohol users. Asian Pac J Cancer Prev. 2012; 13:1633–1637.
Article
6. Chu YH, Tatakis DN, Wee AG. Smokeless tobacco use and periodontal health in a rural male population. J Periodontol. 2010; 81:848–854.
Article
7. Robertson PB, Walsh M, Greene J, Ernster V, Grady D, Hauck W. Periodontal effects associated with the use of smokeless tobacco. J Periodontol. 1990; 61:438–443.
Article
8. Changrani J, Gany FM, Cruz G, Kerr R, Katz R. Paan and Gutka Use in the United States: A Pilot Study in Bangladeshi and Indian-Gujarati Immigrants in New York City. J Immigr Refug Stud. 2006; 4:99–110.
9. Global Adult Tobacco Survey, India 2009-2010. Ministry of Health & Family Welfare Government of India;c2010. cited 2013 Nov 15. Available from: http://mohfw.nic.in/WriteReadData/l892s/1455618937GATS%20India.pdf.
10. Indian Council of Medical Research. National Cancer Registry Programme International Agency for Research on Cancer. VII. Lyon: IARC Scientific Publications;1996.
11. Joshi U, Modi B, Yadav S. A study on prevalence of chewing form of tobacco and existing quitting patterns in urban population of jamnagar, gujarat. Indian J Community Med. 2010; 35:105–108.
Article
12. Anand PS, Kamath KP, Bansal A, Dwivedi S, Anil S. Comparison of periodontal destruction patterns among patients with and without the habit of smokeless tobacco use: a retrospective study. J Periodontal Res. 2013; 48:623–631.
Article
13. Axéll TE. Oral mucosal changes related to smokeless tobacco usage: research findings in Scandinavia. Eur J Cancer B Oral Oncol. 1993; 29B:299–302.
Article
14. Faizuddin M, Bharathi SH, Rohini NV. Estimation of interleukin-1beta levels in the gingival crevicular fluid in health and in inflammatory periodontal disease. J Periodontal Res. 2003; 38:111–114.
Article
15. Noh MK, Jung M, Kim SH, Lee SR, Park KH, Kim DH, et al. Assessment of IL-6, IL-8 and TNF-al crevicular fluid ival tissue of patients with periodontitis. Exp Ther Med. 2013; 6:847–851.
Article
16. Chang MC, Kuo MY, Hahn LJ, Hsieh CC, Lin SK, Jeng JH. Areca nut extract inhibits the growth, attachment, and matrix protein synthesis of cultured human gingival fibroblasts. J Periodontol. 1998; 69:1092–1097.
Article
17. Jeng JH, Lan WH, Hahn LJ, Hsieh CC, Kuo MY. Inhibition of the migration, attachment, spreading, growth and collagen synthesis of human gingival fibroblasts by arecoline, a major areca alkaloid, in vitro. J Oral Pathol Med. 1996; 25:371–375.
Article
18. Chang LY, Wan HC, Lai YL, Liu TY, Hung SL. Enhancing effects of areca nut extracts on the production of interleukin-6 and interleukin-8 by peripheral blood mononuclear cells. J Periodontol. 2006; 77:1969–1977.
Article
19. Chang LY, Wan HC, Lai YL, Kuo YF, Liu TY, Chen YT, et al. Areca nut extracts increased expression of inflammatory cytokines, tumor necrosis factor-alpha, interleukin-1beta, interleukin-6 and interleukin-8, in peripheral blood mononuclear cells. J Periodontal Res. 2009; 44:175–183.
Article
20. Arimilli S, Damratoski BE, Bombick B, Borgerding MF, Prasad GL. Evaluation of cytotoxicity of different tobacco product preparations. Regul Toxicol Pharmacol. 2012; 64:350–360.
21. Johnson GK, Guthmiller JM, Joly S, Organ CC, Dawson DV. Interleukin-1 and interleukin-8 in nicotine- and lipopolysaccharide-exposed gingival keratinocyte cultures. J Periodontal Res. 2010; 45:583–588.
Article
22. Parmar G, Sangwan P, Vashi P, Kulkarni P, Kumar S. Effect of chewing a mixture of areca nut and tobacco on periodontal tissues and oral hygiene status. J Oral Sci. 2008; 50:57–62.
Article
23. Page RC, Eke PI. Case definitions for use in population-based surveillance of periodontitis. J Periodontol. 2007; 78:7 Suppl. 1387–1399.
Article
24. Javed F, Altamash M, Klinge B, Engstrom PE. Periodontal conditions and oral symptoms in gutka-chewers with and without type 2 diabetes. Acta Odontol Scand. 2008; 66:268–273.
Article
25. Silness J, Loe H. Periodontal disease in pregnancy. 3. Response to local treatment. Acta Odontol Scand. 1966; 24:747–759.
26. Loe H, Silness J. Periodontal disease in pregnancy. I. Prevalence and severity. Acta Odontol Scand. 1963; 21:533–551.
Article
27. Gupta PC. Gutka: a major new tobacco hazard in India. Tob Control. 1999; 8:134.
Article
28. Costa FO, Guimaraes AN, Cota LO, Pataro AL, Segundo TK, Cortelli SC, et al. Impact of different periodontitis case definitions on periodontal research. J Oral Sci. 2009; 51:199–206.
Article
29. Javed F, Chotai M, Mehmood A, Almas K. Oral mucosal disorders associated with habitual gutka usage: a review. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010; 109:857–864.
Article
30. Singh GP, Rizvi I, Gupta V, Bains VK. Influence of smokeless tobacco on periodontal health status in local population of north India: a cross-sectional study. Dent Res J (Isfahan). 2011; 8:211–220.
Article
31. Löst C. Depth of alveolar bone dehiscences in relation to gingival recessions. J Clin Periodontol. 1984; 11:583–589.
Article
32. Dahlén G, Naucler C, Nordwall S, Suksu-art N. Oral microflora in betel-chewing adults of the Karen tribe in Thailand. Anaerobe. 2010; 16:331–336.
Article
33. Javed F, Tenenbaum HC, Nogueira-Filho G, Qayyum F, Correa FO, Al-Hezaimi K, et al. Severity of periodontal disease in individuals chewing betel quid with and without tobacco. Am J Med Sci. 2013; 346:273–278.
Article
34. Poore TK, Johnson GK, Reinhardt RA, Organ CC. The effects of smokeless tobacco on clinical parameters of inflammation and gingival crevicular fluid prostaglandin E2, interleukin-1 alpha, and interleukin-1 beta. J Periodontol. 1995; 66:177–183.
Article
35. David J, Yee R, Lama D. The periodontal health of adult Nepalese. Oral Health Prev Dent. 2011; 9:67–81.
36. Giannopoulou C, Kamma JJ, Mombelli A. Effect of inflammation, smoking and stress on gingival crevicular fluid cytokine level. J Clin Periodontol. 2003; 30:145–153.
Article
37. Payne JB, Johnson GK, Reinhardt RA, Maze CR, Dyer JK, Patil KD. Smokeless tobacco effects on monocyte secretion of PGE2 and IL-1 beta. J Periodontol. 1994; 65:937–941.
38. Arimilli S, Damratoski BE, Prasad GL. Combustible and non-combustible tobacco product preparations differentially regulate human peripheral blood mononuclear cell functions. Toxicol In Vitro. 2013; 27:1992–2004.
Article
39. Bascones A, Noronha S, Gomez M, Mota P, Gonzalez Moles MA, Villarroel Dorrego M. Tissue destruction in periodontitis: bacteria or cytokines fault? Quintessence Int. 2005; 36:299–306.
40. Kamma JJ, Giannopoulou C, Vasdekis VG, Mombelli A. Cytokine profile in gingival crevicular fluid of aggressive periodontitis: influence of smoking and stress. J Clin Periodontol. 2004; 31:894–902.
Article
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