J Korean Assoc Maxillofac Plast Reconstr Surg.  2013 May;35(3):155-160.

Salivary Bacterial Counts on Application Time of Oral Antiseptic Agents and Mechanical Irrigation

• Lim HS
• Kim JJ
• Kim M
• Kim HK

Abstract

PURPOSE
The purpose of this study is to establish the acceptable intraoral application time of antiseptic agents and evaluate the effect of mechanical irrigation.
METHODS
A total of 80 subjects were selected for this study. Saliva secreted at the resting state was taken. The subjects were divided into 8 experimental groups, and kept 10% povidone-iodine (PVI) or 0.2% chlorhexidine gluconate (CHX) for 20 or 40 seconds in their oral cavity with/without irrigation of the oral cavity with sterilized normal saline, respectively. Then, the saliva was taken and diluted with phosphate buffered saline and then plated onto 5% sheep blood agar plates, which were incubated. Colony forming unit (CFU) was measured for the salivary bacterial counts.
RESULTS
After application of PVI and CHX, all the experimental groups showed statistically significant decrease in CFU (P<0.01). Group 2 (PVI, 40 s) showed more significant reduction rate in CFU than group 4 (CHX, 40 s; P<0.01). Group 6 (PVI, 40 s, irrigated) showed more significant reduction rate than group 2 (PVI, 40 s; P<0.01). Group 2 (PVI, 40 s) showed more significant reduction rate than group 1 (PVI, 20 s; P<0.01).
CONCLUSION
Application of PVI for 40 seconds and mechanical irrigation with sterilized normal saline showed the best result among the 8 groups in terms of the reduction rate of salivary bacterial counts.

Keyword

Povidone-iodine; Chlorhexidine; Irrigation; Bacteria

MeSH Terms

Agar
Bacteria
Bacterial Load
Chlorhexidine
Mouth
Povidone-Iodine
Saliva
Sheep
Stem Cells
Agar
Chlorhexidine
Povidone-Iodine