J Korean Acad Oral Maxillofac Radiol.  1999 Feb;29(1):327-339.

Experimental Study on the Radiosensitivity and Chemosensitivity of A-431 Cell Line

• Hong SW
• Choi ES
• Koh KJ

Abstract

OBJECTIVES
The purpose of this study was to aid in the prediction of tumor cell tolerance to radiotherapy and/or chemotherapy.
MATERIALS AND METHODS
Human epidermoid carcinoma A-431 cell line were irradiated by 2, 4, 6, 8, 10Gy at a dose rate of 210cGy/min using 60Co Irradiator ALDORADO 8 and then were exposed to bleomycin or cisplatin at concentration of 2mug/mul for 1 hour. The viable cells were determined for each radiation dose and/or each drug at the 4th day and cell surviving curves were obtained using semiautomated MTT assay.
RESULTS
The surviving fraction after irradiation of 2Gy was 0.99, and there was not significant difference of surviving fraction in comparison with the control group on A-431 cell line(p>0.05). But there were significant differences of surviving fractions at doses of 4, 6, 8, 10Gy in comparison with the control group(p<0.05). The cytotoxicity of bleomycin or cisplatin was significantly different in comparison with the control group on A-431 cell line (p<0.05). And the cytotoxicity of cisplatin was greater than that of bleomycin on A-431 cell line (p<0.05). There were significant differences of surviving fractions after irradiation of 2, 4, 6, 8, 10Gy with bleomycin or cisplatin in comparison with each group of irradiation only on A-431 cell line(p<0.05). There were significant differences of surviving fractions between the groups of irradiation with bleomycin and cisplatin at doses of 2, 4Gy(p<0.05), but there were not significant differences of surviving fractions at doses of 6, 8, 10Gy on A-431 cell line (p>0.05).

Keyword

radiosensitivity; chemosensitivity

MeSH Terms

Bleomycin
Carcinoma, Squamous Cell
Cell Line*
Cisplatin
Drug Therapy
Humans
Radiation Tolerance*
Radiotherapy
Bleomycin
Cisplatin