Korean J Vet Res.  2013 Mar;53(1):29-35.

Changes of characterization of Salmonella Typhimurium isolate following sequential exposures to porcine neutrophil

• Lee HS
• Kim A
• Youn M
• Lee JY
• Lim SK
• Kang HY
• Yoo HS
• Park JW
• Wee SH
• Jung SC

Abstract

To develop a live vaccine candidate using an attenuated strain of Salmonella Typhimurium (ST), biochemical properties, plasmid profile, PFGE patterns and pathogenic analysis of the ST isolate were carried out after sequential passage of the ST isolate in porcine neutrophils. By the passage, the ability of the neutrophil-adapted isolate to utilize d-xylose was lost, while the ability of the strain to ferment trehalose was delayed after 2 or more days of the culture. Also, changes including deletion of the gene fragments were observed in PFGE analysis of the neutrophil-adapted isolates. Two plasmids, 105kb and 50kb, were cured in the strain passaged over 15 times in porcine neutrophils. The 50% of lethal dose (LD50) of the parent strain was changed from 1 x 10(5) LD50 to 6 x 10(6) LD50 by the passage in intraperitoneal injection of the strains into mice. These results suggested that bacterial genotypic and phenotypic responses might be globally altered depending on the inside environment of neutrophils.

Keyword

live vaccine; neutrophil; plasmid curing; Salmonella Typhimurium

MeSH Terms

Animals
Humans
Injections, Intraperitoneal
Lethal Dose 50
Mice
Neutrophils
Parents
Plasmids
Salmonella
Salmonella typhimurium
Sprains and Strains
Trehalose
Xylose
Trehalose
Xylose