J Korean Acad Conserv Dent.  2002 Nov;27(6):600-611. 10.5395/JKACD.2002.27.6.600.

In Vitro assessment on viability of human periodontal ligament cells after storage in chlorophyllin-added medium

• Chung WG
• Kim J
• Lee EJ
• Lee SJ

• KMID: 1987318
• DOI: http://doi.org/10.5395/JKACD.2002.27.6.600

Abstract

No abstract available.

Keyword

HBSS; F-medium; ViaSpan; Likorol

MeSH Terms

Adenosine
Allopurinol
Glutathione
Humans
Insulin
Organ Preservation Solutions
Periodontal Ligament
Raffinose
Adenosine
Allopurinol
Glutathione
Insulin
Organ Preservation Solutions
Raffinose

Figure

• 

  Fig. 1 Structure of chlorophyllin (CHL)

• 

  Fig. 2 The mean viability of PDL cells in HBSS at different time periods. The data showed the mean values as the heights of bar.

• 

  Fig. 3 Inverted phase-contrast micrograph (a) of PDL cells stored in HBSS at 24 hours (×100), showing a small number of spindle-shaped fibroblasts. Inverted phase-contrast micrograph (b) of PDL cells stored in HBSS at 72 hours (×100), showing round and detached cells. No viable cells remained.

• 

  Fig. 4 The mean viability of PDL cells following 6-h incubation in different media compared to % of control (HBSS). The data showed the mean values as the heights of the bar plus I bar showing one standard deviation.

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  Fig. 5 The percentage of cells in each of G0-G1, S, G2-M phases of the cell cycle analyzed by flow cytometry. The data showed the mean values as the heights of the bar plus I bar showing one standard deviation.

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  Fig. 6 Cell cycle phase distribution-DNA content histogram and dot-plot profiles by flow cytometric analysis on PDL cells stored up to 6 hours in F-medium, The multicycle mode FIT 2.0 cell cycle software has been used to calculate the fraction of cells in G0-G1 (represented by the first peak on the histogram), S (between the first peak and second peak), and G2-M (the second peak) phases, differing in DNA contents, as shown.

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  Fig. 7 Cell cycle phase distribution-DNA content histogram and dot-plot profiles by flow cytometric analysis on PDL cells stored up to 6 hours in HBSS.

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  Fig. 8 Cell cycle phase distribution-DNA content histogram and dot-plot profiles by flow cytometric analysis on PDL cells stored up to 6 hours in HBSS supplemented with CHL 500 nM.

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