J Korean Acad Conserv Dent.  2003 Mar;28(2):178-183. 10.5395/JKACD.2003.28.2.178.

Identification of putative pathogens in acute endodontic infections by PCR based on 16S rDNA

• Kim JH
• Yoo SY
• Lim SA
• Kook JK
• Lim SS
• Park SH
• Hwang HK

• KMID: 1987246
• DOI: http://doi.org/10.5395/JKACD.2003.28.2.178

Abstract

The purpose of this study was to investigate the frequency of 7 putative pathogens in endodontic infections. The specimens were collected from infected pulpal tissue of patients who were referred for root canal treatment to the department of conservative dentistry, Chosun University. Samples were collected aseptically using a barbed broach and a paper point. The cut barbed broaches and paper points were transferred to an eppendorf tube containing 500 ml of 1 X PBS. DNAs were extracted from the samples by direct DNA extraction method using lysis buffer (0.5% EDTA, 1% Triton X-100). Identification of 7 putative pathogens was performed by PCR based on 16S rDNA. The target species were as follows: Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Bacteroides forsythus, Actinobacillus actinomycetemcomitans, and Treponema denticola. Our data revealed that the prevalence of P. endodontalis was found in 88.6% (39/54), P. gingivalis 52.3% (23/44), P. nigrescens 18.2% (8/44), P. intermedia 15.9% (7/44), B. forsythus 18.2% (8/44), A. actinomycetemcomitans 2.3% (1/44), T. denticola 25% (11/44) of the samples. The high prevalence of P. endodontalis and P. gingivalis suggests that they may play an important role in the etiology of endodontic infections.

Keyword

16S rDNA; Acute endodontic infections; PCR; Pathogens

MeSH Terms

Aggregatibacter actinomycetemcomitans
Bacteroides
Dental Pulp Cavity
Dentistry
DNA
DNA, Ribosomal*
Edetic Acid
Humans
Neptune
Polymerase Chain Reaction*
Porphyromonas endodontalis
Porphyromonas gingivalis
Prevalence
Prevotella intermedia
Prevotella nigrescens
Treponema denticola
DNA
DNA, Ribosomal
Edetic Acid

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