Korean J Med.  2000 Aug;59(2):132-141.

Polymorphisms of PPARgamma2 gene in patients with type 2 diabetes mellitus and obesity

• Oh SJ
• Kang SY
• Kim YS
• Kim DY
• Kim SW
• Yang IM
• Kim JW
• Choi YK
• Park HS
• Paeng JR

Abstract

BACKGROUND
Peroxisome proliferator activated receptor-gamma (PPAR-gamma) is a nuclear receptor that regulate adipocyte differentiation and modulate intracellular insulin-signaling events. As such, PPARgamma is a candidate gene for several human disorders including obesity and type 2 diabetes mellitus. The objective of our study was to examine the relationship between genetic variation of PPARgamma2 and diabetes and obesity in Korean subjects.
METHODS
We studied 99 subjects with type 2 diabetes mellitus, 128 obesity patients and 97 controls. Screening for mutation at codon 12 and 115 of PPARgamma2 were carried out by PCR-RFLP analyses. Statistical significance was evaluated by Chi-square test.
RESULTS
The allele frequency of the Pro12Ala PPARgamma2 variant were 0.05 in controls, 0.06 in type 2 diabetes group, and 0.07 in obesity group (p=0.47). Pro115Gln variant were only proline homozygote in all groups. Genotype frequencies were also similar and conformed to expectations of the Hardy-Weinberg rule. The presence of PPARgamma2 gene variant was no associated with concentrations of total cholesterol, triglyceride, HDL-cholesterol, and also with fasting glucose.
CONCLUSION
We concluded that the Pro12Ala and Pro115Gln PPARgamma2 missense mutation may not be associated with type 2 diabetes mellitus and obesity in Korean patients.

Keyword

Liver cirrhosis; Biliary; Antimitochondrial antibody; Anti-M2 antibody; Enzyme-linked Immunosorbent assay

MeSH Terms

Adipocytes
Cholesterol
Codon
Diabetes Mellitus, Type 2*
Enzyme-Linked Immunosorbent Assay
Fasting
Gene Frequency
Genetic Variation
Genotype
Glucose
Homozygote
Humans
Liver Cirrhosis
Mass Screening
Mutation, Missense
Obesity*
Peroxisomes
PPAR gamma*
Proline
Triglycerides
Cholesterol
Codon
Glucose
PPAR gamma
Proline