Korean J Gynecol Oncol Colposc.
2003 Dec;14(4):300-305.
Changes of Gene Expression between HaCat and SiHa cells using GeneFishingTM PCR
- Affiliations
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- 1Catholic Research Institutes of Medical Science, College of Medicine, The Catholic University of Korea.
- 2Department of Obstetrics and Gynecology, College of Medicine, The Catholic University of Korea.
- 3College of Pharmacy, Seoul National University.
Abstract
OBJECTIVE
The purpose of this study is investigated the differentially expressed genes between normal and cervical cancer cell line.
METHODS
We used normal human keratinocyte (HaCaT) as a control and HPV-16 positive cervical cancer (SiHa) cell line. Two cell lines were studied differential expressed genes by using GeneFishingTM PCR and analyzed with BLAST search.
RESULTS
As compared with normal, cervical cancer cell line was showed 25 up-regulated genes including the S1-2-2, S5-1 and 24 down-regulated genes.
CONCLUSION
GeneFishTM PCR test is very sensitive and effective method for detection of changed gene expression. We could search differentially expressed genes between normal and cervical cancer cell line. In the future, we need to research various genes function to participate in the process of tumor development and progression.
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