Ewha Med J.  1982 Sep;5(3):117-124. 10.12771/emj.1982.5.3.117.

The Electron Microscopic study of the Synapses of the Locus Coeruleus in the Squirrel Monkey, Saimiri sciureus

• Lee HL
• Kim SH

• KMID: 1969221
• DOI: http://doi.org/10.12771/emj.1982.5.3.117

Abstract

This study provides a description of the normal morphology of the synapses of the locus coeruleus in the squirrel monkey, Saimiri sciureus, at the electron microscopic level. The animals were perfused with 2% glutaraldehyde-1% paraformaldehyde fixative in phosphate buffer at pH 7.4. Tissues from the locus coeruleus were postfixed by 2% osmium tetroxide in phosphate buffer at pH 7.4, and dehydrated in graded ethanol and aceton, and embedded in Epon 812. The polymerization was carried out in oven at 60degrees C. The ultrathin sections were cut on a LKB ultratome, contrasted with uranyl acetate and lead citrate, and examined in a Siemens Elmiskop 1A electron microscope. Special attention during the observation was given to the different types of synapses. Five types of synapses with many characteristics, could be differentiated : small-round vesicle terminals(52%), pleomorphic vesicle terminals(25%), elongated vesicle terminals(16%), small-round and dense core vesicle terminals(5%) and large-round vesicle terminals(2%). Some of the axosomatic synapses with small round or pleomorphic vesicles make an indirect contact to the soma by somatic appendages.

MeSH Terms

Animals
Carisoprodol
Citric Acid
Ethanol
Hydrogen-Ion Concentration
Locus Coeruleus*
Osmium Tetroxide
Polymerization
Polymers
Saimiri*
Sciuridae*
Secretory Vesicles
Synapses*
Carisoprodol
Citric Acid
Ethanol
Osmium Tetroxide
Polymers