Ewha Med J.  1984 Jun;7(2):71-78. 10.12771/emj.1984.7.2.71.

Reduction in Binding of Aflatoxin B1 to Rat Liver DNA by Vitamin A and E Pretreatment

• Hong YS

• KMID: 1967556
• DOI: http://doi.org/10.12771/emj.1984.7.2.71

Abstract

We examined pattern of covalent modification of DNA produced in rat liver after exposure to single dose of aflatoxin B1. DNA-bound aflatoxin B1 adducts obtained from rat liver microsomes were hydrolyzed with weak acid to yield 2, 3-dihydro-3-hydroxy-(N7-guanyl) aflatoxin B1 as major product. This adduct was derived from the aflatoxin B1, 2, 3-dioxide and accounted for approximately 80% of the carcinogen-derived radioactivity incorporated into DNA. Acid hydrolysis of the nucleic acid-aflatoxin B1, adducts also yielded 2,3-dihydro-2,3-dihydroxy-aflatoxin B1 and other minor products. Vitamin A and E pretreatment of rats resulted in reduction in the level of hepatic AFB1-DNA adducts to two-third of the control value. Therefore, administration of vitamin A and E to rats chages in AFB1 metabolism probably decreased modification of DNA during this experiment.

MeSH Terms

Aflatoxin B1*
Aflatoxins*
Animals
DNA*
Hydrolysis
Liver*
Metabolism
Microsomes, Liver
Radioactivity
Rats*
Vitamin A*
Vitamins*
Aflatoxin B1
Aflatoxins
DNA
Vitamin A
Vitamins