J Korean Assoc Oral Maxillofac Surg.  2004 Oct;30(5):400-405.

Anti-angiogenic activity of genistein in oral carcinogenesis

Affiliations
  • 1Department of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University, Korea. seungilsong@hanmail.net
  • 2Department of Dentistry, School of Medicine, Ajou University, Korea.

Abstract

Angiogenesis inhibition is major concern to cancer chemotherapy and many studies about compound inhibiting angiogenesis is in progression. The long-known preventive effect of plant-based diet on tumorigenesis and other chronic diseases is well documented. Especially soy extract, genistein, is known to be potent angiogenesis inhibitor and prevent development and progression of tumor. In the present study, the effect of angiogenesis on tumorigenesis and chemopreventive effect of genistein by angiogenesis inhibition in hamster buccal pouch oral carcinigenesis model induced by 7.12-dimethylbenza(a)nthracene (DMBA) was studied. Forty eight Syrian Golden young adult hamsters (150-200 gm) were divided into two groups. In control group, 0.5% DMBA in heavy mineral oil was applied to hamster buccal pouch three times a week and in experimental group, 0.1 mg of genistein is administered orally everyday in addition to DMBA application. The animals were euthanized from 2 weeks to 16 weeks with interval of 2 week. HandE staining and immunohistochemistry was performed to evaluate microvessel density by using factor VIII-related antigen and avidin-biotin technique. Microvessels per area was quantified and compared between control and experimental group statistically. The results were as follows. 1. Microvessel density was increased time dependently in both groups and especially the increase was significant from 12 weeks to 16 weeks. 2. When comparing both group, the experimental group showed significantly low microvessel density than control group in 12 weeks (p=0.043), 14 weeks (p=0.050), 16 weeks (p=0.037). Based on these results, it was concluded that genistein influenced oral carcinogenesis by angiogenesis inhibition.

Keyword

Angiogenesis; Genistein; DMBA; Immunihistochemistry; Oral carcinogenesis

MeSH Terms

9,10-Dimethyl-1,2-benzanthracene
Animals
Carcinogenesis*
Chronic Disease
Cricetinae
Diet
Drug Therapy
Genistein*
Hand
Humans
Immunohistochemistry
Microvessels
Mineral Oil
von Willebrand Factor
Young Adult
9,10-Dimethyl-1,2-benzanthracene
Genistein
Mineral Oil
von Willebrand Factor
Full Text Links
  • JKAOMS
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
    DB Error: unknown error