J Korean Assoc Maxillofac Plast Reconstr Surg.  1999 Nov;21(4):325-331.

Development of bone regenerating material using bone morphogenetic protein(rhBMP-2) and bioresorbable polymer

• Lee JH
• Kim JW
• Ahn KM
• Kim KK
• Lee ZH

Abstract

We tested the bone regenerating capacity and histologic response of bioresorbable matrix-type implant, which was made with Poly(lactide-co-glycolide)(PLGA) and bone apatite for the carrier of bone morphogenetic protein(BMP). The critical size defect of 8 mm in diameter was created at the calvaria of SD rats(n=18), and repaired with polymer implant with 15 microgram of rhBMP-2(n=9) or without it(n=9). At 2 weeks, 1 month and 3 months after implantation, the animals were sacrificed(3 animals at every interval and group) and histologically evaluated. The calvarial defect which was repaired with polymer with BMP healed with newly formed bone about 70% of total defect. But that without BMP showed only 0 to under 30% bony healing. Inflammatory response was absent in both group through the experimental period, but there's marked foreign body giant response though it was a little less significant in polymer with BMP group. As the polymer was resorbed, the space was infiltrated and replaced by fibrovascular tissue, not by bone. In conclusion, our formulation of bioresorbable matrix implant loaded with bone morphogenetic protein works good as a bone regenerating material. However, it is mandatory to devise our system to have better osteoinductive and osteoconductive property, and less multinucleated giant cell response.

MeSH Terms

Animals
Bone Morphogenetic Proteins
Foreign Bodies
Giant Cells
Polymers*
Skull
Bone Morphogenetic Proteins
Polymers