J Korean Ophthalmol Soc.  1964 Nov;5(2):37-39.

The Effects of Alpha-Chymotrypsin and Trypsin on the Rabbit's Cornea

• Kang NY

Abstract

The posterior surface of the rabbit's cornea was scraped by a spatula or incised by a cystotome, followed by injection of alpha-chymotrypsin or trypsin into the anterior chamber. The concentration of enzymes used were 5mg/cc and O.5mg/cc of alpha-chymotrypsin and 5.000 Anson unit/cc and 500unit/ cc of trypsin. Three minutes after the introduction of enzymes into the anterior chamber, irrigation of the anterior chamber with normal saline was performed. The cornea was examined macroscopically and biomicroscopica11y for about 3 weeks. Histological examination was done on the 3rd and the 11th post-operative days. The cornea was affected more intensively and extensively when the stroma was injured than when only the endothelium was injured. Alpha-chymotrypsin was more toxic to the cornea than trypsin and led sometimes to a corneal perforation.

MeSH Terms

Anterior Chamber
Cornea*
Corneal Perforation
Endothelium
Trypsin*
Trypsin