J Korean Soc Microbiol.  1997 Apr;32(2):213-218.

Species-Specific Detection of Listeria monocytogenes by Polymerase Chain Reaction

• Yang BS
• Park JS

Abstract

Three Listeria monocytogenes strains isolated from clinical cerebrospinal fluid samples. A PCR was performed by using oligonuclotide primers derived from the invasion-associated protein(iap) related gene sequence. As a result of PCR, we obtained 287-bp DNA fragment of specific product to L. monocytogenes species. The chromsomal DNA of genera Listeria species were subjected to southern blot hybridization with HRP-labeled 287-bp DNA probe to produce a L. monocytogenes banding pattern. In the cases of 10-fold dilution of L. monocytogenes cells, 8 x 10(2) cells/ml were detected by PCR.

Keyword

iap gene; Listeria monocytogenes; HRP; nested PCR

MeSH Terms

Blotting, Southern
Cerebrospinal Fluid
DNA
Listeria monocytogenes*
Listeria*
Polymerase Chain Reaction*
DNA