J Korean Vasc Surg Soc.  1998 Apr;14(1):18-22.

Differential Protein Expression of Neointima after Autologous Vein Grafting in Mongrel Dogs

• Kim DI
• Lee SY
• Han JS
• Lee BB

Abstract

Arterial grafts using autologous vein as conduits are common, being used for coronary, femoral, and tibial artery occlusive disease. They have been clearly demonstrated to have a superior patency rate when compared with prosthetic conduits in small sized vessels. Although they provide an overall better conduit, they still may suffer the same problems as their prosthetic counterparts, namely, development of neointimal hyperplasia and ultimately restenosis. Numerous studies have demonstrated that vascular smooth muscle cell(VSMC) plays a significant role in the development of neointimal hyperplasia regardless of where they develop after angioplasty, vein bypass grafting, or prosthetic grafting. In order to find proteins differentially expressed in VSMC from bypassed veins and normal veins, total protein extracts were separated by SDS-PAGE and differentially expressed bands were identified. The results were summerized as follows; (1) 173, 110, 89, 39, 36, 33 KDa proteins were differentially expressed in bypassed veins compared with normal veins. (2) 2 proteins(173, 110 KDa) of these were also expressed in normal arteries. (3) Therefore, vein grafts were adapted in arterial environment via vascular hyperplasia in which protein compositions of vessel walls resembled arteries. In conclusion, neointimal hyperplasia after autologous vein bypass seemed to be an adaptative response that vein grafts encountered arterial hemodynamics underwent vascular remodeling to reduce the high distensibility of veins and to decrease wall stress to normal arterial levels.

Keyword

Protein; Graft; Neointima; Bypass; Electrophoresis

MeSH Terms

Angioplasty
Animals
Arteries
Dogs*
Electrophoresis
Electrophoresis, Polyacrylamide Gel
Hemodynamics
Hyperplasia
Muscle, Smooth, Vascular
Neointima*
Tibial Arteries
Transplants*
Veins*