Korean J Anesthesiol.  2007 Apr;52(4):449-453. 10.4097/kjae.2007.52.4.449.

Expression of Neuronal Nitric Oxide Synthase (nNOS) on Ischemia/reperfusion Injury in Rat Spinal Cord

• Kwon HU
• Lee KH
• Kang PS
• Cho CK
• Yang CW
• Yang CM
• Park JT

• KMID: 1937574
• DOI: http://doi.org/10.4097/kjae.2007.52.4.449

Abstract

BACKGROUND
The aim of this experimental study was to investigate the expression of nNOS following ischemia/reperfusion injury in rat spinal cord.
METHODS
Spraque-Dawley rats (250-300 gm) were classified into two groups according to experimental methods. Control group (n = 5) received sham operation. Ischemia in experimental group (n = 5) was induced by clamping the abdominal aorta just below the left renal artery and the abdominal aorta just above the aortic bifurcation for 20 minutes followed by 20 minutes reperfusion. The spinal cord was obtained 7 days after operation. The expression of nNOS was examined in gray and white matter with confocal microscope and Image Analyzer.
RESULTS
There is no difference in nNOS expression of nNOS between gray and white matter in control group. Comparing to control group, experimental group showed higher levels of nNOS expression (P < 0.05).
CONCLUSIONS
nNOS expression increased in the rat spinal cord after ischemia/reperfusion injury. We suggest that overproduction of NO may play a role in progressive nerve cell damage.

Keyword

ischemia/reperfusion injury; nNOS; spinal cord

MeSH Terms

Animals
Aorta, Abdominal
Constriction
Ischemia
Neurons*
Nitric Oxide Synthase Type I*
Rats*
Renal Artery
Reperfusion
Spinal Cord*
Nitric Oxide Synthase Type I