Korean J Dermatol.  2007 Apr;45(4):327-331.

Expression of Protoporphyrin IX Induced by Liposome delta-5-aminolevulinic Acid in Cultured Sebocytes and Hair Organs

• Lee WJ
• Lee SJ
• Kim DW
• Park KD
• Sohn MY
• Kim MK
• Kim JC
• Han IS

Abstract

BACKGROUND: Photodynamic therapy with topical delta-5-aminolevulinic acid (ALA) has been introduced for effective treatment of facial acne. Topical routes for ALA have been explored via a photosensitizer delivery system.
OBJECTIVE
We performed this study to evaluate absorption of liposome ALA (Lipo-ALA) into the sebocytes of cultured sebaceous glands and hair organs.
METHODS
We cultured KGM sebocytes that were derived from human sebaceous glands. In addition, we cultured William E media pilosebaceous hair units that were isolated from the occipital scalp. Lipo-ALA (10 microgram/ml) and ALA (10 microgram/ml) were added to the 1 ml KGM containing sebocytes for 24 hours and the 1 ml William E media containing pilosebaceous hair units for 6, 24, 48 and 72 hours, respectively.
RESULTS
Absorption of Lipo-ALA into the sebocytes of cultured sebaceous gland and hair organs was higher than that of ALA.
CONCLUSION
Lipo-ALA may be considered as an effective photosensitizer in photodynamic therapy for facial acne.

Keyword

Acne; ALA; Liposome delta-5-aminolevulinic acid; Photodynamic therapy

MeSH Terms

Absorption
Acne Vulgaris
Hair*
Humans
Liposomes*
Photochemotherapy
Scalp
Sebaceous Glands
Liposomes