Korean J Otolaryngol-Head Neck Surg.  1997 Mar;40(3):402-408.

Neurogenic Inflammation Induced by Hypertonic Saline in Rat Nasal Mucosa

• Lee HM
• Lee SH

Abstract

The purpose of this study was to investigate whether inhaled hypertonic aerosols could increase vascular permeability in the rat nasal mucosa which was mediated by the release of sensory neuropeptides. Sprague Dawley rats were divided into following groups: hypertonic saline(3.6-14.4%NaCl) inhalation group, phosphoramidon(2.5mg/kg, i.v.) pretreated group, capsaicin desensitized group, substance P inhalation(10-4 M) group with or without capsaicin pretreatment group. Rats were exposed to ultrasonically nebulized aerosols. The magnitude of the increase in vascular permeability of nasal mucosa was observed using fluorescent microscope and measured by quantifying the extravasation of Evans blue dye. Hypertonic saline aerosols increased vascular permeability dose-dependently compared with 0.9% NaCl(p<0.05). The neutral endopeptidase inhibitor, phosphoramidon potentiated the increase of vascular permeability(p<0.01) and desensitization of sensory nerve endings by pretreatment with capsaicin reduced the usual increase in vascular permeability induced by 3.6% NaCl(p<0.01). However, the increase in vascular permeability induced by aerosolized substance P was unchanged(p>0.05). Therefore, these results suggest that aerosols of hypertonic saline increase the plasma extravasation by stimulating the release of neuropeptides from the sensory nerves in the rat nasal mucosa.

Keyword

Neurogenic inflammation; Hypertonic saline; Neuropeptide

MeSH Terms

Aerosols
Animals
Capillary Permeability
Capsaicin
Evans Blue
Inhalation
Nasal Mucosa*
Neprilysin
Neurogenic Inflammation*
Neuropeptides
Plasma
Rats*
Rats, Sprague-Dawley
Sensory Receptor Cells
Substance P
Aerosols
Capsaicin
Evans Blue
Neprilysin
Neuropeptides
Substance P