Korean J Urol.
1989 Feb;30(1):1-6.
Transitional Cell Tumor Culture and Chemosensitivity Test: Preliminary Report
- Affiliations
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- 1Department of Urology, Hallym University, College of Medicine, Korea.
- 2Department of Pathology, Hallym University, College of Medicine, Seoul, Korea.
Abstract
- Usually, tumor stem cells have been assessed functionally in animals with various in vivo transplantation assays or with in vitro colony forming assays. Such studies indicate the tumor stem cell assays can be used to study the properties of tumor stem cells and to delineate the difference in individual sensitivities to chemotherapeutic agents. Over the past year (1987,5-1988,4) we attempted to culture the epithelial tumor cells in laboratory to test the chemosensitivity to Methotrexate, cytarabine, and daunorubicin in different concentrations. The in vitro chemosensitivity of these cells to anticancer drugs was disappointed in much. Only 14% of the cultured cells were inhibited the growth more than 50% in over-all.
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