The Experimental Study for Effect of Prostaglandin on Detrusor Muscle

Kim, Suk Chool; Yoon, Jong Byung

Abstract

In this investigation, the bladder of guinea pig was longitudinally mounted in the muscle chamber and contracted by electrical field stimulation(EFS). It was purposed to elucidate the physiological significance of prostaglandin(PG) on the neurotransmitter release from nerve ending. 1. The inhibitory response of 5 x 0.0000001 g/ml atropine was significant only by high frequency (p<0.05) However. the curve of frequency-response were shifted to left upward by 5 x 0.00000001 g/ml phentolamine, it were not significant. Because the contraction was completely abolished by 0.00000001 and 0.0000001g/ml tetrodotoxin, it was evident that the responses to electrical stimulation are entirely due to nerve mediated excitation. 2. The curve of frequency-response were significantly shifted to left upward by 5x 0.00000001 and 5x0.0000001 g/ml arachidonic acid(p<0.05). Though the contraction were inhibited by 5x0.0000001g/ml atropine it was not significant. 3. The curves of frequency-response showed dose-response relation by 5x 0.00000001, 5 x0.000000001 and 5x 0.0000000001g/ml PGE2. The contraction was significant only by high frequency in 5x0.0000000001g/ml PGE. and the frequency-response curves were significant in varying frequencies in 5x0.000000001and 5x0.00000001g/ml PGE2(p<0.05). 4. Although the contraction was found by 5x0.0001g/ml aspirin, the curves of frequency-response were not significant. The contraction by 5x 0.0001g/ml aspirin and 5x0.000001/ml arachidonic acid was not significant too.