Korean J Gastroenterol.  2002 Mar;39(3):161-166.

Effect of Helicobacter pylori Infection on Mucosal Interleukin-8 Expression in Gastric Metaplasia

• Oh YS
• Hong SJ
• Kim JO
• Cho JY
• Lee JS
• Lee MS
• Jin SY
• Shim CS

Abstract

BACKGROUND/AIMS: Gastric metaplasia (GM) is found in many patients with duodenal ulcer, and the recurrence of duodenal ulcer is likely to be associated with GM as well as H. pylori infection. This study was performed to evaluate the effect of H. pylori infection on histologic findings and IL-8 expression of the gastroduodenal mucosa including the area of GM in the patients with duodenal ulcer scar.
METHODS
Thirty patients with duodenal ulcer scar and nine volunteers with no gastroduodenal ulcer as control underwent gastroduodenoscopy with methylene blue spray and biopsies. The biopsy specimens were stained with HandE, Warthin-Starry, and alcian blue-PAS, and then measured their interleukin-8 expression (IL-8) by ELISA.
RESULTS
Control group had no GM. Thirty patients with duodenal ulcer scar showed GM. Among them, the grade of inflammation in patients with H. pylori infection was significantly higher than those without H. pylori infection (p<0.05). On the area of GM, IL-8 level was significantly higher in H. pylori-infected patients than in H. pylori-eradicated patients or controls. The IL-8 level in the area of GM was correlated with the grade of inflammation (r=0.573, p<0.05) and antral H. pylori density (r=0.506, p<0.05).
CONCLUSIONS
H. pylori infection seems to be a part of the pathogenesis of duodenal ulcer by inducing inflammation and IL-8 expression in the area of GM.

Keyword

Helicobacter pylori; Duodenal ulcer; Gastric metaplasia; IL-8

MeSH Terms

Biopsy
Cicatrix
Duodenal Ulcer
Enzyme-Linked Immunosorbent Assay
Hand
Helicobacter pylori*
Helicobacter*
Humans
Inflammation
Interleukin-8*
Metaplasia*
Methylene Blue
Mucous Membrane
Peptic Ulcer
Recurrence
Volunteers
Interleukin-8
Methylene Blue