Korean J Blood Transfus.
1999 May;10(1):21-26.
Rh C/c, E/e genotyping by polymerase chain reaction using sequence-specific primers
- Affiliations
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- 1Blood Transfusion Research Institute, The Republic of Korea National Red Cross.
- 2Department of Clinical Pathology, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea.
Abstract
- BACKGROUND
S: With the recent elucidation of genetic basis of Rh blood group, it is now available the molecular genotyping methods for Rh blood typing. These can be used when serological typing is difficult. This study was conducted to investigate the usefulness of Rh genotyping method for Koreans.
METHODS
Genotyping for Rh C/c and E/e was performed in peripheral blood DNA samples from 34 blood donors by polymerase chain reaction using sequence-specific primers (PCR-SSP). The PCR determined genotypes were compared with serologically determined phenotypes.
RESULTS
The Rh C/c and E/e genotyping results of 34 blood donors were full concordance with the results of their serologic phenotyping.
CONCLUSIONS
Rh genotyping method on the basis of Rh genetic model can be applied to Koreans. This genotyping method would be useful tool in prenatal Rh typing of fetus at risk of hemolytic disease and when serotyping is not available for example massive transfusion.
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