Korean J Pathol.  2010 Jun;44(3):234-242.

Expression of Raf-1 Kinase Inhibitory Protein in Extrahepatic Bile Duct Carcinoma

• Kim HS
• Kim GY
• Lim SJ
• Kim YW

Abstract

BACKGROUND
Raf-1 kinase inhibitory protein (RKIP) recently has been identified as a metastasis suppressor in a variety of human carcinomas. The prognostic significance of RKIP expression in extrahepatic bile duct (EBD) carcinoma has not been studied. The aims of the current study were to evaluate RKIP expression and to determine the prognostic significance of RKIP expression in EBD carcinoma.
METHODS
Immunohistochemical staining for RKIP was performed for 131 cases of EBD carcinoma. The associations of RKIP expression with clinicopathologic parameters and patient outcomes were examined. Multivariate logistic regression analysis was used to identify independent predictive parameters for lymphovascular invasion and nodal and distant metastases.
RESULTS
Loss of RKIP expression was observed in 55.0% (72/131) of cases. EBD carcinoma had significantly lower RKIP immunoreactivity than normal EBD (p < 0.001). Loss of RKIP expression was significantly associated with lymphatic invasion (p = 0.030) and nodal metastasis (p = 0.036), but it was not found to be a significant prognostic predictor for overall, disease-free or distant metastasis-free survival. In addition, loss of RKIP expression was an independent predictor for lymphatic invasion (p = 0.027).
CONCLUSIONS
These results suggest that RKIP may play a role in the suppression of lymphatic invasion and nodal metastasis in EBD carcinoma.

Keyword

Raf-1 kinase inhibitor protein; Immunohistochemistry; Bile duct neoplasms; Lymphatic metastasis

MeSH Terms

Bile Duct Neoplasms
Bile Ducts, Extrahepatic
Humans
Immunohistochemistry
Logistic Models
Lymphatic Metastasis
Neoplasm Metastasis
Proto-Oncogene Proteins c-raf
Proto-Oncogene Proteins c-raf