Clin Exp Reprod Med.  2015 Mar;42(1):14-21. 10.5653/cerm.2015.42.1.14.

The parental origin correlates with the karyotype of human embryos developing from tripronuclear zygotes

• Joergensen MW
• Labouriau
• Hindkjaer
• Stougaard
• Kolevraa S
• Bolund L
• Agerholm I
• Sunde L

• KMID: 1800421
• DOI: http://doi.org/10.5653/cerm.2015.42.1.14

Abstract

OBJECTIVE
It has previously been suggested that embryos developing from intracytoplasmic sperm-injected (ICSI) zygotes with three pronuclei (3PN) are endowed with a mechanism for self-correction of triploidy to diploidy. 3PN are also observed in zygotes after conventional in vitro fertilization (IVF). The parental origin, however, differs between the two fertilization methods. Whereas the vast majority of 3PN IVF zygotes are of dispermic origin and thus more likely to have two centrioles, the 3PN ICSI zygotes are digynic in origin and therefore, more likely to have one centriole. In the present study, we examine whether the parental origin of 3PN embryos correlates with the karyotype.
METHODS
The karyotype of each nucleus was estimated using four sequential fluorescence in situ hybridizations-each with two probes-resulting in quantitative information of 8 different chromosomes. The karyotypes were then compared and correlated to the parental origin.
RESULTS
3PN ICSI embryos displayed a significantly larger and more coordinated reduction from the assumed initial 3 sets of chromosomes than 3PN IVF embryos.
CONCLUSION
The differences in the parental origin-and hence the number of centrioles-between the 3PN IVF and the 3PN ICSI zygotes are likely to be the cause of the differences in karyotypes.

Keyword

Centrioles; Embryos; Human; Ploidy; Preimplantation; Tripronuclear

MeSH Terms

Centrioles
Diploidy
Embryonic Structures*
Fertilization
Fertilization in Vitro
Fluorescence
Humans
Karyotype*
Parents*
Ploidies
Sperm Injections, Intracytoplasmic
Triploidy
Zygote*