J Korean Med Sci.  2014 Sep;29(9):1266-1270. 10.3346/jkms.2014.29.9.1266.

Anti-Mullerian Hormone Levels in the Follicular Fluid of the Preovulatory Follicle: A Predictor for Oocyte Fertilization and Quality of Embryo

• Kim JH
• Lee JR
• Chang HJ
• Jee BC
• Suh CS
• Kim SH

• KMID: 1794606
• DOI: http://doi.org/10.3346/jkms.2014.29.9.1266

Abstract

This prospective study investigated the relationship between anti-Mullerian hormone (AMH) level in the follicular fluid (FF) and the quality of the oocyte and embryo. A total of 65 FF samples from 54 women were included in this study. FF was collected from the largest preovulatory follicle sized> or =20 mm of mean diameter from each ovary. Samples were divided into 3 groups according to the FF AMH levels: below the 33th percentile (low group, FF AMH<2.1 ng/mL, n=21), between the 33th and the 67th percentile (intermediate group, FF AMH=2.1-3.6 ng/mL, n=22), and above the 67th percentile (high group, FF AMH>3.6 ng/mL, n=22). The quality of the ensuing oocytes and embryos was evaluated by fertilization rate and embryo score. FF AMH levels correlated positively with the matched embryo score on day 3 after fertilization (r=0.331, P=0.015). The normal fertilization rate was significantly lower in the low group than in the intermediate group (61.9% vs. 95.5% vs. 77.3%, respectively, P=0.028). Our results suggest that the FF AMH level could be a predictor of the ensuing oocyte and embryo quality.

Keyword

Anti-Mullerian Hormone; Follicular Fluid; Oocyte Competence; Embryo Quality; In Vitro Fertilization

MeSH Terms

Adult
Anti-Mullerian Hormone/*analysis
Embryo, Mammalian/*cytology
Female
Fertilization in Vitro
Follicular Fluid/*metabolism
Humans
Oocytes/cytology
Prospective Studies
Anti-Mullerian Hormone

Figure

• 

  Fig. 1 Correlation of the follicular fluid (FF) anti-Müllerian hormone (AMH) level with day 3 embryo score.

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  Fig. 2 Fertilization and top-quality embryo rate in low, intermediate, and high anti-Müllerian hormone (AMH) groups. *P % 0.05 when compared with the low group (chi-square test).

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