Korean J Parasitol.  2013 Oct;51(5):563-567.

Molecular Diagnosis of an Ocular Toxocariasis Patient in Vietnam

• Van De N
• Trung NV
• Duyet LV
• Chai JY

Abstract

An ocular Toxocara canis infection is reported for the first time in Vietnam. A 34-year-old man residing in a village of Son La Province, North Vietnam, visited the National Eye Hospital (NEH) in August 2011. He felt a bulge-sticking pain in his left eye and loss of vision occurred over 3 months before visiting the hospital. The eye examination in the hospital showed damage of the left eye, red eye, retinal fibrosis, retinal detachment, inflammation of the eye tissues, retinal granulomas, and a parasitic cyst inside. A larva of Toxocara was collected with the cyst by a medical doctor by surgery. Comparison of 264 nucleotides of internal transcribed spacer 2 (ITS2) of ribosomal DNA was done between our Vietnamese Toxocara canis and other Toxocara geographical isolates, including Chinese T. canis, Japanese T. canis, Sri Lankan T. canis, and Iranian T. canis. The nucleotide homology was 97-99%, when our T. canis was compared with geographical isolates. Identification of a T. canis infection in the eye by a molecular method was performed for the first time in Vietnam.

Keyword

Toxocara canis; case report; eye; molecular diagnosis; PCR; ITS2; Vietnam

MeSH Terms

Adult
Animals
Base Sequence
DNA, Helminth/chemistry/genetics
DNA, Ribosomal/chemistry/genetics
DNA, Ribosomal Spacer/chemistry/genetics
Eye Infections, Parasitic/*diagnosis/parasitology
Humans
Larva
Male
Molecular Sequence Data
Phylogeny
Sequence Alignment
Sequence Analysis, DNA
Toxocara canis/classification/genetics/*isolation & purification
Toxocariasis/*diagnosis/parasitology
Vietnam
DNA, Helminth
DNA, Ribosomal
DNA, Ribosomal Spacer