Ann Lab Med.  2014 May;34(3):259-262. 10.3343/alm.2014.34.3.259.

The First Known Case of Blood Group Chimerism in Monochorionic Dizygotic Twins in Korea

• Lee OJ
• Cho D
• Shin MG
• Kim SO
• Park JT
• Kim HK
• Ryang DW

• KMID: 1791932
• DOI: http://doi.org/10.3343/alm.2014.34.3.259

Abstract

No abstract available.

MeSH Terms

ABO Blood-Group System/immunology
Agglutination Tests
Alleles
Base Sequence
Blood Group Antigens/*genetics/immunology
Chimerism
Exons
Female
Genotype
Humans
Infant
Male
Microsatellite Repeats/genetics
Pedigree
Republic of Korea
Sequence Analysis, DNA
Twins, Dizygotic
ABO Blood-Group System
Blood Group Antigens

Figure

• 

  Fig. 1 Blood groups of the propositus and his family. The phenotypes and genotypes of the propositus' family members (except his twin sister) were normal. Mixed blood of types B and O, caused by the genotype B101/O01/O02 (as revealed by cloning and direct sequencing of exons 6 and 7) was observed in the propositus (arrow) and his twin sister.

• 

  Fig. 2 Results of the monoclonal gel test showing two populations of blood cells on typing of the propositus. A mixed-field agglutination pattern was observed by using the anti-B reagent (arrows), indicating the simultaneous presence of two red blood cell populations (Twin, male, A). The same pattern was observed in the twin sister of the propositus (Twin, Female, B).Abbreviations: DVI, RhDVI variants; ctl, control.

• 

  Fig. 3 Nucleotide sequences in the region of nucleotides 261 (arrow), 297, 646, and 681 in ABO exons 6 and 7. The top chromatogram was produced by routine sequencing of exons 6 and 7. The presence of both O01- and O02-allele-specific nucleotides and B-allele-specific nucleotides (arrow) is evident. The bottom three chromatograms were obtained via sequencing conducted after cloning, and conclusively show B101-, O01-, and O02-allele-specific nucleotides.Abbreviation: nt, nucleotide.

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