Yonsei Med J.  2009 Feb;50(1):132-136. 10.3349/ymj.2009.50.1.132.

Polymorphism of Haptoglobin in Patients with Premature Rupture of Membrane

• Cho JK
• Kim YH
• Park IY
• Shin JC
• Oh MK
• Park SJ
• Kim NH
• Kim IS

• KMID: 1782978
• DOI: http://doi.org/10.3349/ymj.2009.50.1.132

Abstract

PURPOSE
To investigate whether allelic polymorphism of haptoglobin (Hp) is associated with premature rupture of membrane (PROM), the Hp phenotypes of pregnant women with PROM were analyzed.
PATIENTS AND METHODS
The Hp phenotypes of 221 pregnant Korean women (187 control and 34 PROM patients) were determined by benzidine/hydrogen peroxide staining, following native polyacrylamide gel electrophoresis of hemoglobin-mixed sera. The Hp allele frequencies were calculated from the data of Hp phenotypes, and overall association with PROM was evaluated using Pearson Chi-Square test.
RESULTS
The polymorphic distribution of the patients cohort who underwent a normal delivery (control group) was similar to that of healthy Koreans. In contrast, however, patients with PROM showed significantly higher occurrence of the Hp 1-1 phenotype than control group (23.5% vs 8.0%). Hp 2-2 phenotype was lower in PROM cohort (38.2%) than in the control group (48.7%). The Hp1 allele frequency in PROM group was significantly higher than that in the control group (0.426 vs 0.297, p = 0.034) with odds ratio of 1.762 (95% CI: 1.038 - 2.991).
CONCLUSION
These findings suggest that pregnant Korean women who possess Hp1 allele (expressed as Hp 1-1 phenotype) have higher incidence of PROM than women with Hp2 allele (expressed as Hp 2-2 phenotype). This is the first study that evaluated the significance of Hp polymorphism with respect to the development of PROM.

Keyword

Haptoglobin; genetic polymorphism; phenotype; premature rupture of membrane (pregnancy)

MeSH Terms

Adult
Asian Continental Ancestry Group/*statistics & numerical data
Female
Fetal Membranes, Premature Rupture/*ethnology/*genetics
Gene Frequency
Genetic Predisposition to Disease/ethnology
Haptoglobins/*genetics
Humans
Incidence
Infant, Newborn
Korea/epidemiology
Phenotype
*Polymorphism, Genetic
Pregnancy

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