Korean J Lab Med.  2007 Oct;27(5):318-323. 10.3343/kjlm.2007.27.5.318.

Reconfirmation of Preanalytical Variables and Establishment of Reference Intervals of Platelet Function Analyzer-100 Closure Times in Korean Adults

• Cho YU
• Chi HS
• Jang S
• Park CJ

• KMID: 1781529
• DOI: http://doi.org/10.3343/kjlm.2007.27.5.318

Abstract

BACKGROUND: Platelet Function Analyzer-100 (PFA-100, Dade-Behring, Germany) is an instrument that simulates in vivo hemostatic plug formation under high shear flow by measuring the time required to occlude aperture. We performed this study to reconfirm preanalytical variables and establish the reference intervals of Korean adults. METHODS: A total of 120 healthy individuals were enrolled. Closure times (CT) with the collagen/ epinephrine (CEPI) and the collagen/ADP (CADP) cartridges were measured. RESULTS: The reference intervals by the central 95th percentile were 82-182 sec for CEPI-CT and 62-109 sec for CADP-CT. Females had significantly longer CEPI- and CADP-CT than males (P=0.034 and 0.022, respectively). Individuals over 40 yr showed shorter CEPI- and CADP-CT compared with younger ones (P=0.002 and 0.003, respectively). CEPI- and CADP-CT values measured in the afternoon were significantly longer than corresponding ones in the morning (P<0.0001 in both conditions). Group O blood groups were related to longer CEPI- and CADP-CT compared with non-O blood groups (P=0.0003 and <0.0001, respectively). CADP-CT was weakly correlated with hematocrit (r=-0.296, P=0.001), but not CEPI-CT. CONCLUSIONS: We reconfirmed the preanalytical variables and established the reference intervals of PFA-100 CT in Korean adults. It is recommended that reference interval of this test should be verified according to age, diurnal variation, and ABO blood groups for optimal utilization.

Keyword

PFA-100 closure time; Korean adult; Reference interval; Preanalytical variables

MeSH Terms

ABO Blood-Group System
Adult
Female
Humans
Korea
Male
Middle Aged
Platelet Function Tests/*instrumentation/standards
Reference Values
Regression Analysis
Time Factors

Figure

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  Fig. 1. Schematic diagram of the mechanism of the platelet function analyzer-100 (from the operating manual of platelet function analyzer-100).

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  Fig. 2. Distribution of closure time values determined in the morning tested with the collagen/epinephrine (A) and the collagen/ADP (B) cartridge. The reference intervals (central 95 percentile) are 82-182 sec for collagen/epinephrine and 62-109 sec for collagen/ADP.

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  Fig. 3. Linear regression analysis revealed an inverse correlation between age and CEPI-CT (A) and between hematocrit and CADP-CT (B).

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