Korean J Lab Med.  2007 Apr;27(2):77-82. 10.3343/kjlm.2007.27.2.77.

JAK2(V617F) Mutation in Korean Patients with Essential Thrombocythemia

• Ahn JY
• Yoo SJ
• Bang SM
• Park PW
• Seo YH
• Shin DB
• Lee JH

• KMID: 1781463
• DOI: http://doi.org/10.3343/kjlm.2007.27.2.77

Abstract

BACKGROUND: Essential thrombocythemia (ET) is thought to reflect transformation of a multipotent hematopoietic stem cell, but its molecular pathogenesis remains obscure. But tyrosine kinase, especially Janus kinase 2 (JAK2), has been implicated in myeloproliferative disorders other than chronic myeloid leukemia. We investigated the frequency of JAK2 mutation and its correlation with other clinicopathologic variables in Korean patients with ET and reactive thrombocytosis (RT). METHODS: JAK2 mutation analysis was performed on genomic DNA from bone marrow aspirates of 24 patients with ET and peripheral blood in 36 patients with RT using allele-specific PCR. RESULTS: JAK2 mutation was detected in 11 patients (46%) among the 24 patients with ET and was not found in 36 patients with RT. In patients with ET, older age and leukocytosis were related with JAK2 mutation without statistical significance (P=0.172 and 0.094, respectively). But this mutation was not correlated with sex, hemoglobin, platelet count, splenomegaly, increased cellularity of bone marrow, bone marrow fibrosis and vascular complications. CONCLUSIONS: The current observation strengthens the specific association between JAK2 mutation and ET. At the diagnosis of ET in Korean patients, identification of JAK2 mutation should be incorporated in the basis for new approaches.

Keyword

JAK2 mutation; Essential thrombocythemia; Reactive thrombocytosis

MeSH Terms

Aged
Alleles
Amino Acid Substitution
Blood Platelets/metabolism
Female
Humans
Janus Kinase 2/*genetics
Korea
Male
Middle Aged
Polymerase Chain Reaction
Thrombocytosis/blood/*diagnosis/genetics

Figure

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  Fig. 1. Allele-specific PCR in patients with ET and RT. The 364 bp of PCR product is an internal control and 203bp is an allele specific product. Lane SM, molecular size marker; lane 1, 2, and 7, positive results from ET patients; lane 4, 5, 6, negative results from RT patients; lane 3, negative control. Abbreviations: ET, essential thrombocytopenia; RT, reactive thrombocytosis.

Reference

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