Clin Exp Reprod Med.  2011 Dec;38(4):210-215. 10.5653/cerm.2011.38.4.210.

Lower growth factor expression in follicular fluid undergone in-vitro fertilization

• Han M
• Park SB
• Park BJ

• KMID: 1743677
• DOI: http://doi.org/10.5653/cerm.2011.38.4.210

Abstract

OBJECTIVE
This study was performed to identify whether growth and differentiation factor-9 (GDF-9) and transforming growth factor-beta1 (TGF-beta1) expressions would be lower in the follicular fluid (FF) of those over age 35 who underwent IVF than under age 35.
METHODS
A total of 24 IVF cycles (20 patients) were included in this study. All of patients were stimulated for IVF by the GnRH short protocol and divided into two groups for analysis, according to their age: <35 group (14 cycles, 11 patients) vs. > or =35 group (10 cycles, 9 patients). The expression levels of GDF-9 and TGF-beta1 were determined by western blotting and quantitative enzyme-linked immunosorbent assay.
RESULTS
The numbers of retrieved oocytes and metaphase II oocytes were significantly lower in the > or =35 group. Lower expression of GDF-9 and TGF-beta1 by western blotting in the > or =35 group were observed as well. The mean GDF-9 and TGF-beta1 levels by enzyme-linked immunosorbent assay were lower in the > or =35 group. The values were 6,850.5+/-928.4 ng/L vs. 3,333.3+/-1,089.2 ng/L of GDF-9 (p<0.05) and 3,844.1+/-571.1 ng/L vs. 2,187.7+/-754.0 ng/L of TGF-beta1 (p<0.05). A negative correlation between GDF-9 and age was observed (r=-0.546, p=0.006).
CONCLUSION
GDF-9 and TGF-beta1 production from stimulated ovaries during IVF appears to decrease with age.

Keyword

Follicular fluid; Growth and differentiation factor-9; Transforming growth factor-beta1; Human

MeSH Terms

Blotting, Western
Enzyme-Linked Immunosorbent Assay
Female
Fertilization
Follicular Fluid
Gonadotropin-Releasing Hormone
Growth Differentiation Factor 9
Humans
Metaphase
Oocytes
Ovary
Transforming Growth Factor beta1
Gonadotropin-Releasing Hormone
Growth Differentiation Factor 9
Transforming Growth Factor beta1