Clin Exp Reprod Med.  2011 Dec;38(4):186-192. 10.5653/cerm.2011.38.4.186.

Application of two different synthetic sequential media for the human IVF-ET program: a prospective, randomized, and comparative study

• Yoon J
• Yoon HJ
• Juhn KM
• Ko JK
• Yoon SH
• Ko Y
• Lim JH

• KMID: 1743674
• DOI: http://doi.org/10.5653/cerm.2011.38.4.186

Abstract

OBJECTIVE
Since IVF program was first established, various types of media and culture systems have been developed either in-house or commercially. The aim of this study was to compare the efficacy of in-house Maria Research Center (MRC) media to that of commercially available Sydney IVF media in human day 3 embryo transfer cycles.
METHODS
Three hundred sixty nine couples were included in this prospective, randomized, and comparative study. All couples undergoing IVF treatment at the Maria Fertility Hospital were randomly assigned to either Sydney IVF (n=178) or MRC (n=191) media.
RESULTS
No difference was observed between the MRC media and Sydney IVF media groups with respect to fertilization rate (74.4% vs. 75.5%). The clinical pregnancy and implantation rates of MRC media (47.1% and 20.0%, respectively) were also similar to those of Sydney IVF media (44.4% and 19.4%, respectively). However, the proportion of embryos with good quality on day 3 was significantly higher in the MRC media group than the Sydney IVF media group (50.2% vs. 43.2%) (p<0.05).
CONCLUSION
MRC media were as effective as Sydney IVF media for sustaining embryo development and pregnancy rates. The present study implies that MRC media can be a suitable alternative to commercially available media for human IVF-ET program.

Keyword

MRC media; Sydney IVF media; Day 3 embryo transfer; In vitro fertilization-embryo transfer; Human

MeSH Terms

Embryo Transfer
Embryonic Development
Embryonic Structures
Family Characteristics
Female
Fertility
Fertilization
Humans
Pregnancy
Pregnancy Rate
Prospective Studies