Korean J Ophthalmol.  1996 Dec;10(2):68-75. 10.3341/kjo.1996.10.2.68.

Effect of topically applied Na-hyaluronan on experimental corneal alkali wound healing

• Chung JH
• Kim HJ
• Fagerholmb P
• Cho BC

• KMID: 1735161
• DOI: http://doi.org/10.3341/kjo.1996.10.2.68

Abstract

The effect of topically applied 1% sodium hyaluronate (Na-HA) on the healing of a standardized corneal alkali wound was studied. The healing of the epithelium, stroma, and endothelium was evaluated separately, using quantitative methods. Central corneal alkali wound was produced in one eye of the rabbits by applying a 5.5-mm round filter paper, soaked in 1 N NaOH, for 60 seconds. 1% Na-HA in the treatment group and phosphate buffered saline (PBS) in the control group were given topically 4 times per day for 2 days, 1- and 3-weeks. Epithelial and endothelial healing was assessed morphometrically from standardized photographs and micrographs, respectively. Stromal healing was determined by counting polymorphonuclear leukocytes (PMN) and keratocytes in the central and marginal wound areas. A positive healing influence was observed in the epithelium. In stromal healing, 1% Na-HA treated corneas showed less PMNs and more keratocytes than the control group, suggesting that topically applied 1% Na-HA may suppress the stromal PMN infiltration and enhance the keratocyte repopulation during corneal alkali wound healing. However, no significant difference was found in morphometric evaluation of endothelial healing between the two groups.

Keyword

hyaluronan; cornea; alkali wound; epithelium; strorna; keratocyte; PMN; endothelium; rabbit

MeSH Terms

Administration, Topical
Animals
Burns, Chemical/*drug therapy/etiology/pathology
Cell Count
Cornea/*drug effects/pathology
Corneal Stroma/drug effects/pathology
Endothelium, Corneal/drug effects/pathology
Epithelium/drug effects/pathology
Eye Burns/chemically induced/*drug therapy/pathology
Hyaluronic Acid/administration & dosage/*pharmacology
Ophthalmic Solutions
Rabbits
Sodium Hydroxide/toxicity
Wound Healing/*drug effects
Hyaluronic Acid
Ophthalmic Solutions
Sodium Hydroxide