Korean J Ophthalmol.  1991 Dec;5(2):68-75. 10.3341/kjo.1991.5.2.68.

Cellular components of proliferative vitreoretinal membranes

• Kwak SI
• Chung H
• Lee J

• KMID: 1735082
• DOI: http://doi.org/10.3341/kjo.1991.5.2.68

Abstract

To understand the pathogenesis of proliferative vitreoretinal membrane formation which occurs in proliferative vitreoretinopathy (PVR) and proliferative diabetic retinopathy (PDR), etc., accurate identification of the cellular components of the membrane is needed. This study was performed to identify cellular components of the membranes by means of immunohistochemical technique. 11 proliferative vitreoretinal membranes which were surgically obtained from 7 eyes with PVR and 4 eyes with PDR were stained with monoclonal antibodies against cytokeratin, glial fibrillary acidic protein (GFAP), or vimentin using immunoperoxidase technique (ABC method). In the PVR membranes, mean cell positivities for cytokeratin, GFAP and vimentin were 48%, 1% and 92%, respectively and in the PDR membranes, 0%, 5% and 93%, respectively. The above results suggest that retinal pigment epithelial cells and fibroblasts are major cellular components of PVR membranes, and that mesenchymal cells are major cellular components and glial cells are minor cellular components of PDR membranes.

Keyword

cellular component; immunohistochemical technique; proliferative diabe-tic retinopathy; proliferative vitreoretinopathy

MeSH Terms

Adolescent
Adult
Antibodies, Monoclonal
Cell Membrane/metabolism/pathology
Diabetic Retinopathy/metabolism/pathology
Eye Diseases/metabolism/pathology
Female
Humans
Immunoenzyme Techniques
Intermediate Filament Proteins/*analysis
Male
Middle Aged
Retinal Diseases/metabolism/*pathology
Vitreous Body/metabolism/*pathology
Antibodies, Monoclonal
Intermediate Filament Proteins