Korean J Ophthalmol.  1987 Dec;1(2):90-94. 10.3341/kjo.1987.1.2.90.

Fluorescent low density lipoprotein and acetylated low density lipoprotein labeling of cultured bovine trabecular endothelial cells

• Ahn BH
• Oh MJ
• Bae HB

• KMID: 1735006
• DOI: http://doi.org/10.3341/kjo.1987.1.2.90

Abstract

A preliminary study was performed to investigate the staining characteristics of trabecula. endothelial cells with low density lipoprotein (LDL) and acetylated low density lipoprotein (Ac-LDL) labeled with a fluorescent probe, 1, 1`- dioctadecyl-3,3,3`, 3`- tetramethyl-indocarbocyanine perchlorate (Dil). Trabecular endothelial cells revealed a strong fluorescence with Dil-LDL, which was contradictory to the previous results obtained from other types of endothelial cells. These cells also showed a moderate fluorescence with Dil-Ac-LDL. Scleral fibroblasts and keratocytes showed a moderate to strong fluorescence with Dil-LDL and a weak fluorescence with Dil-Ac-LDL. Corneal endothelial cells revealed a very weak background fluorescence with Dil-LDL and a moderate fluorescence with Dil-Ar-LDL. Therefore, these four kinds of cells could not be definitely differentiated depending only on the staining characteristics with Dil-LDL and Dil-Ac-LDt.

Keyword

low density lipoprotein; acetylated low density lipoprotein; trabecular endothelial cell; tissue culture; Dil-LDL; Dil-Ac-LDL

MeSH Terms

Animals
Cattle
Endothelium/cytology
Fluorescent Dyes/*diagnostic use
Lipoproteins, LDL/*metabolism
Trabecular Meshwork/*metabolism
Fluorescent Dyes
Lipoproteins, LDL