Korean J Parasitol.  2004 Dec;42(4):169-174. 10.3347/kjp.2004.42.4.169.

Organ-specific antigens of Clonorchis sinensis

• Li S
• Chung BS
• Choi MH
• Hong ST

• KMID: 1720486
• DOI: http://doi.org/10.3347/kjp.2004.42.4.169

Abstract

This study was carried out to find out specific proteins from different organs of Clonorchis sinensis. Crude extract, organ-specific and excretory-secretory (ES) proteins were analyzed by immunoblot with infected human sera. The bands of 7- and 17-kDa were main component of intestinal fluid and ES protein and commonly found in all organspecific proteins. The 17-kDa protein was observed from ES antigen, intestinal fluid, eggs and sperms, 26- and 28- kDa proteins were from the uterus, vitellaria, and ovary, and 34-, 37-, 43- and 50-kDa proteins were mainly from the testis and sperms. Serum of mice immunized with sperms reacted to the 50-kDa protein by immunoblotting and immunohistochemical staining showed a positive reaction at the seminal receptacle and seminiferous tubule. The present results show that the 7-kDa protein is a common antigen of every part or organ of C. sinensis, but different organs express their specific antigenic protein bands.

Keyword

Clonorchis sinensis; organ-specific antigen; immunoblot; 7-kDa antigen

MeSH Terms

Animals
Antigens, Helminth/*analysis
Clonorchis sinensis/anatomy & histology/*immunology
Female
Humans
Male
Mice
Mice, Inbred BALB C
Rabbits
Research Support, Non-U.S. Gov't