Korean J Parasitol.
2011 Sep;49(3):245-254. 10.3347/kjp.2011.49.3.245.
Parasitic Helminth Cystatin Inhibits DSS-Induced Intestinal Inflammation Via IL-10+F4/80+ Macrophage Recruitment
- Affiliations
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- 1Department of Parasitology, School of Medicine, Pusan National University, Yangsan 626-813, Korea. hsyu@pusan.ac.kr
- 2Healthy Jang's Internal Medicine, Busan 612-804, Korea.
- 3Department of Parasitology, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 660-751, Korea.
- 4Department of Microbiology and Immunology, School of Medicine, Pusan National University, Yangsan 626-813, Korea.
- 5Department of Nursing, College of Nursing, Pusan National University, Busan 626-813, Korea.
- KMID: 1715647
- DOI: http://doi.org/10.3347/kjp.2011.49.3.245
Abstract
- Many immune down-regulatory molecules have been isolated from parasites, including cystatin (cystain protease inhibitor). In a previous study, we isolated and characterized Type I cystatin (CsStefin-1) of the liver fluke, Clonorchis sinensis. To investigate whether the CsStefin-1 might be a new host immune modulator, we induced intestinal inflammation in mice by dextran sodium sulfate (DSS) and treated them with recombinant CsStefin-1 (rCsStefin-1). The disease activity index (DAI) increased in DSS only-treated mice. In contrast, the DAI value was significantly reduced in rCsStefin-1-treated mice than DSS only-treated mice. In addition, the colon length of DSS only-treated mice was shorter than that of rCsStefin-1 treated mice. The secretion levels of IFN-gamma and TNF-alpha in the spleen and mesenteric lymph nodes (MLNs) were significantly increased by DSS treatment, but the level of TNF-alpha in MLNs was significantly decreased by rCsStefin-1 treatment. IL-10 production in both spleen and MLNs was significantly increased, and IL-10+F4/80+ macrophage cells were significantly increased in the spleen and MLNs of rCsStefin-1 treated mice after DSS treatment. In conclusion, rCsStefin-1 could reduce the intestinal inflammation occurring after DSS treatment, these effects might be related with recruitment of IL-10 secreting macrophages.
Keyword
MeSH Terms
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Animals
Antigens, Differentiation/analysis
Clonorchis sinensis/*enzymology
Colon/pathology
Cystatins/*metabolism
Cytokines/secretion
Dextran Sulfate/toxicity
Female
Helminth Proteins/*metabolism
Immunologic Factors/*metabolism
Inflammation/chemically induced/*pathology
Interleukin-10/analysis
Intestines/*drug effects/pathology
Lymph Nodes/immunology
Macrophages/chemistry/*immunology
Mice
Mice, Inbred C57BL
Severity of Illness Index
Spleen/immunology
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