Korean J Parasitol.
1992 Mar;30(1):25-31. 10.3347/kjp.1992.30.1.25.
Immunohistochemical localization of 36 and 29 kDa proteins in sparganum
- Affiliations
-
- 1Department of Parasitology, College of Medicine, Chung-Ang University, Seoul, Korea.
- KMID: 1715530
- DOI: http://doi.org/10.3347/kjp.1992.30.1.25
Abstract
- Antigenic proteins of 36 and 29 kDa were localized in Spirometra mansoni plerocercoid (sparganum) immunohistochemically by avidin biotin complex (ABC) staining. When polyclonal antibodies such as BALB/c mouse serum immunized with crude saline extract of sparganum or confirmed sparganosis sera were reacted as primary antibodies, the positive chromogen (3-amino, 9-ethylcarbazole) reactions were recognized at syncytial tegument, tegumental cells, muscle and parenchymal cells and lining cells of excretory canals. A monoclonal antibody (MAb) which was reacting to 36 and 29 kDa proteins in the extract of the worm was localized at the syncytial tegument and tegumental cells. The present results suggested that the potent antigenic proteins of 36 and 29 kDa in sparganum were produced at the tegumental cells and transported to the syncytial tegument.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Single step purification of potent antigenic protein from sparganum by gelatin-affinity chromatography
- Modification of carbohydrate compositions of 31/36 kDa proteins of plerocercoids (sparganum) of Spirometra mansoni grown in different intermediate hosts
- Antigen specificity of 36 and 31 kDa proteins of Spirometra erinacei plerocercoid in tissue invading nematodiasis
- Antigenic protein fractions reacting with sera of sparganosis patients
- Component proteins and protease activities in excretory-secretory product of sparganum
