- Single step purification of potent antigenic protein from sparganum by gelatin-affinity chromatography
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Kong Y, Kang SY, Cho SY
- KMID: 1715566
- Korean J Parasitol.
- 1991 Mar;29(1):1-7.
- doi: 10.3347/kjp.1991.29.1.1
Out of many component proteins in crude saline extract of Spirometra mansoni plerocercoid (sparganum), 36 kDa and 29 kDa proteins were found to be the most antigenic and were already... -
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- Protein composition and antigenicity of the tegument from Paragonimus westermani
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Kim SI, Cho SY
- KMID: 1715471
- Korean J Parasitol.
- 1993 Sep;31(3):269-276.
- doi: 10.3347/kjp.1993.31.3.269
To ascertain that tegument of Paragonimus westermani has specific antigenic proteins, the tegumental fraction was isolated from 10-month-old worms by 0.1% digitonin solution, and subjected to SDS-PAGE and immunoblot. Component... -
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- Purification of antigenic protein of sparganum by immunoaffinity chromatography using a monoclonal antibody
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Cho SY, Kang SY, Kong Y
- KMID: 1720301
- Korean J Parasitol.
- 1990 Sep;28(3):135-142.
- doi: 10.3347/kjp.1990.28.3.135
The quality improvement of antigen (crude saline extract) of Spirometra mansoni pleroceroid (sparganum) was investigated by protein purification. The crude extract was fractionated by gel filtration through Sephacryl S-300 Superfine.... -
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- Antigen specificity of 36 and 31 kDa proteins of Spirometra erinacei plerocercoid in tissue invading nematodiasis
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Morakote N, Kong Y
- KMID: 1715487
- Korean J Parasitol.
- 1993 Jun;31(2):169-171.
- doi: 10.3347/kjp.1993.31.2.169
Diagnostic specificity of 36 and 31 kDa proteins of Spirometra erinacei plerocercoid (sparganum) was evaluated by micro-ELISA in tissue invading nematodiasis such as 25 gnathostomiasis, 33 angiostrongyliasis, 22 trichinellosis patients,... -
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- Immune reactions between excretory-secretory antigens and specific antibodies of Clonorchis sinensis before and after praziquantel treatment in experimentally infected rabbits
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Kim SI
- KMID: 1715455
- Korean J Parasitol.
- 1994 Mar;32(1):35-42.
- doi: 10.3347/kjp.1994.32.1.35
This study was designed to evaluate the humoral immune reactions in clonorchiasis before and after praziquantel treatment. Rabbits were infected with 150 or 450 metacercariae, treated on 4 and 8... -
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- Immunohistochemical localization of 36 and 29 kDa proteins in sparganum
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Kim LS, Kong Y, Kang SY, Cho SY
- KMID: 1715530
- Korean J Parasitol.
- 1992 Mar;30(1):25-31.
- doi: 10.3347/kjp.1992.30.1.25
Antigenic proteins of 36 and 29 kDa were localized in Spirometra mansoni plerocercoid (sparganum) immunohistochemically by avidin biotin complex (ABC) staining. When polyclonal antibodies such as BALB/c mouse serum immunized... -
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- Immunohistochemical observation on the antigens inducing IgG and IgM antibodies against sparganum
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Kim CH, Choi WS
- KMID: 1715538
- Korean J Parasitol.
- 1991 Dec;29(4):339-353.
- doi: 10.3347/kjp.1991.29.4.339
Localization and characterization of the antigenic components of sparganum which induced IgG and IgM antibodies in the host were studied by immunohistochemical techniques and SDS-PAGE and Western blotting. The antigen... -
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- Tissue origin of soluble component proteins in saline extract of adult Paragonimus westermani
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Kong Y, Park CY, Kang SY, Cho SY
- KMID: 1044811
- Korean J Parasitol.
- 1992 Jun;30(2):91-100.
- doi: 10.3347/kjp.1992.30.2.91
Tissue origin of individual component proteins in crude extract of adult Paragonimus westermani was investigated. Major soluble component proteins were separated by disc-PAGE in 8% slab gel. By predefined Rf... -
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- Cross-reacting and specific antigenic components in cystic fluid from metacestodes of Echinococcus granulosus and Taenia solium
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Kong Y, Kang SY, Cho SY, Min DY
- KMID: 1720276
- Korean J Parasitol.
- 1989 Jun;27(2):131-139.
- doi: 10.3347/kjp.1989.27.2.131
Sera from confirmed patients of 5 hydatidosis, 67 neurocysticercosis and 89 other parasitic diseases were tested for specific antibody (IgG) levels by ELISA to cystic fluid antigens from metacestodes of... -
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- Antigenic protein fractions of Metagonimus yokogawai reacting with patient sera
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Lee SC, Chung YB, Kong Y, Kang SY, Cho SY
- KMID: 1715494
- Korean J Parasitol.
- 1993 Mar;31(1):43-48.
- doi: 10.3347/kjp.1993.31.1.43
Antibody test is sometimes necessary for the diagnosis of acute human metagonimiasis because eggs may not be detected in stool. The antibody test (ELISA) was evaluated for its significance by... -
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- Blastogenesis of splenic lymphocytes to specific antigens and PHA in Paragonimus westermani infected mice
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Min DY, Shin MH, Choi R
- KMID: 1715532
- Korean J Parasitol.
- 1992 Mar;30(1):43-48.
- doi: 10.3347/kjp.1992.30.1.43
Paragonimus westermani is a common fluke in Korea. The present study aimed to observe the cell mediated immune response in experimental paragonimiasis of mice. The mouse (BALB/c) was orally inoculated... -
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- Analysis of antigenic specificities of Paragonimus westermani developmental stages using immunoblot technique
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Joo KH, Hong SC, Chung MS, Rim HJ
- KMID: 1720261
- Korean J Parasitol.
- 1989 Mar;27(1):1-7.
- doi: 10.3347/kjp.1989.27.1.1
Serodiagnosis of parasitic infections is widely used, since parasites or their eggs are not always detected by ordinary methods. The sensitive tests such as ELISA are highly dependent on the... -
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- ELISA of rat sera infected with Paragonimus iloktsuenensis
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Lim BK, Lee OR, Nam HS
- KMID: 1720308
- Korean J Parasitol.
- 1990 Dec;28(4):207-212.
- doi: 10.3347/kjp.1990.28.4.207
Enzyme-linked immunosorbent assay (ELISA) of paragonimiasis iloktsuenensis rat sera was performed using crude antigens of Paragonimus iloktsuenensis (PIA), P. westermani (PWA) and Clonorchis sinensis (CSA). Three crude antigens (PIA, PWA,... -
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- Antigenic localities in the tissues of Paragonimus westermani by developmental stages using immunogold labeling method
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Rim HJ, Kim SJ, Sun IJ, Lee JS
- KMID: 1715527
- Korean J Parasitol.
- 1992 Mar;30(1):1-14.
- doi: 10.3347/kjp.1992.30.1.1
In order to observe the antigenic localization in the tissues of Paragonimus westermani of developmental stages, immunogold labeling method was applied using serum of the cats which were infected with... -
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- A study on the body fluid antigen of Clonorchis sinensis using immunogold labeling method
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Chu BD, Rim HJ, Kim SJ
- KMID: 1720294
- Korean J Parasitol.
- 1990 Mar;28(1):11-23.
- doi: 10.3347/kjp.1990.28.1.11
In order to observe the antigenic localization in the tissues of the adult Clonorchis sinensis, immunogold labeling method was applied using serum immunoglobulins (IgG) of either worm-infected rabbits (group I)... -
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- Antigenic localities in the tissues of Metagonimus yokogawai in the period of growth
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Rim HJ, Kim SJ, Yang MG
- KMID: 1715510
- Korean J Parasitol.
- 1992 Dec;30(4):309-321.
- doi: 10.3347/kjp.1992.30.4.309
In order to observe the antigenic localization in the tissues of Metagonimus yokogawai in growth stages, immunogoldlabeling method was applied to using serum of the cat which infected with isolated... -
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- Antigenic localities in the tissues of the young adult worm of Paragonimus westermani using immunogold labeling method
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Kwon OS, Lee JS, Rim HJ, Kim SJ
- KMID: 1715569
- Korean J Parasitol.
- 1991 Mar;29(1):31-41.
- doi: 10.3347/kjp.1991.29.1.31
In order to observe the antigenic localization in the tissues of the young adult Paragonimus westermani, immunogold labeling method was applied using serum immunoglobulins(IgG) of the dog which infected with... -
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- Antigenic localities in the tissues of Metagonimus yokogawai observed by immunogoldlabeling method
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Ahn H, Rim HJ, Kim SJ
- KMID: 1715550
- Korean J Parasitol.
- 1991 Sep;29(3):245-257.
- doi: 10.3347/kjp.1991.29.3.245
In order to determine the antigenic localization in the tissues of the adult Metagonimus yokogawai, immunogoldlabeling method was applied using serum immunoglobulins(IgG) of cats which were infected with isolated metacercariae... -
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- Analysis of Clonorchis sinensis antigens and diagnosis of clonorchiasis using monoclonal antibodies
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Yong TS, Im K, Chung PR
- KMID: 1715554
- Korean J Parasitol.
- 1991 Sep;29(3):293-310.
- doi: 10.3347/kjp.1991.29.3.293
Clonorchis sinensis is a common parasite of man in Korea. Researches on the specific antigens of C. sinensis would be valuable not only because those elucidate the molecular characteristics of... -
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- Demonstration of species-specific and cross reactive components of Paragonimus westermani crude worm antigen by EITB
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Joo KH, Ahn H, Chung MS, Rim HJ
- KMID: 1720262
- Korean J Parasitol.
- 1989 Mar;27(1):9-14.
- doi: 10.3347/kjp.1989.27.1.9
Enzyme-linked immunoelectrotransfer blot (EITB) using crude worm antigen of adult Paragonimus westermani was performed for human patients sera to identify the species-specific components. Crude antigen was obtained by homogenizing and... -
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