Korean J Parasitol.  1997 Dec;35(4):295-298. 10.3347/kjp.1997.35.4.295.

Different RAPD patterns between Metagonimus yokogawai and Metagonimus Miyata type

• Yu JR
• Chung JS
• Chai JY

• KMID: 1715318
• DOI: http://doi.org/10.3347/kjp.1997.35.4.295

Abstract

Genomic DNA from Metagonimus yokogawai and Metagonimus Miyata type was amplified by polymerase chain reaction based on the random amplification of polymorphic DNA (RAPD) technique. Eight random 10-mer oligonucleotide primers (OPA-02, 5-TGCCGAGCTG-3; OPA-09, 5-GGGTAACGCC-3; OPA-10, 5-GTGATCGCAG-3; OPA-11, 5-CAATCGCCGT-3; OPA-13, 5-CAGCACCCAC-3; OPA-17, 5-GACCGCTTGT-3; OPA-19, 5-CAAACGTCGG-3; OPA-20, 5-GTTGCGATCC-3) with a G + C content of 60-70% (Kit A, Operon Technologies Inc., California, USA) could produce distinguishable banding patterns between the two Metagonimus species. From the results of this study, it was suggested that Metagonimus Miyata type has a different DNA sequence from M. Yokogawai.

MeSH Terms

Animal
Base Sequence
DNA Primers
DNA, Helminth/genetics
DNA, Helminth/analysis*
Genome
Heterophyidae/genetics*
Heterophyidae/classification
Polymerase Chain Reaction/methods