J Korean Med Sci.  2010 May;25(5):663-670. 10.3346/jkms.2010.25.5.663.

Human Umbilical Cord Blood Mononuclear Cell Transplantation in Rats with Intrinsic Sphincter Deficiency

• Lim JJ
• Jang JB
• Kim JY
• Moon SH
• Lee CN
• Lee KJ

• KMID: 1713947
• DOI: http://doi.org/10.3346/jkms.2010.25.5.663

Abstract

To evaluate the effectiveness of the human umbilical cord blood (HUCB) transplantation for the treatment of intrinsic sphincter deficiency (ISD), we analyzed the short term effects of HUCB mononuclear cell transplantation in rats with induced-ISD. ISD was induced in rats by electro-cauterization of periurethral soft tissue with HUCB mononuclear cell injection after 1 week. The sphincter function measured by mean leak point pressure was significantly improved in the experimental group compared to the control group at 4 weeks. (91.75+/-18.99 mmHg vs. 65.02+/-22.09 mmHg, P=0.001). Histologically, the sphincter muscle was restored without damage while in the control group it appeared markedly disrupted with atrophic muscle layers and collagen deposit. We identified injected HUCB cells in the tissue sections by Di-I signal and Prussian blue staining. HUCB mononuclear cell injection significantly improved urethral sphincter function, suggesting its potential efficacy in the treatment of ISD.

Keyword

Urinary Incontinence, Stress; Intrinsic Sphincter Deficiency; Leak Point Pressure; Human Umbilical Cord Blood Mononuclear Cells; Stem Cells

MeSH Terms

Animals
Cells, Cultured
Cord Blood Stem Cell Transplantation/*methods
Humans
Leukocytes, Mononuclear/*transplantation
Rats
Rats, Sprague-Dawley
Treatment Outcome
Urinary Incontinence, Stress/diagnosis/*physiopathology/*surgery
Urologic Surgical Procedures/*methods

Figure

• 

  Fig. 1 Comparative effect of HUCB mononuclear cell injection on leak point pressure between groups at 2 and 4 weeks. *is significantly different from each other (P<0.05) at each time. Compared with control group, the leak point pressure in the experimental group at 4 week after HUCB mononuclear cell injection was significantly increased.

• 

  Fig. 2 Photographs show Feridex®-labeled HUCB mononuclear cells in urethral tissue at 2 weeks post-injection. A number of cells considered human cells within the tissue sections were detected by blue-colored signals. (A, C) Control group; (B, D) Experimental group (Magnification ×200, 400).

• 

  Fig. 3 Photographs show Di-I labeled HUCB mononuclear cells in urethral tissue at 2 weeks post-injection. A number of cells considered human cells within the tissue sections were detected by red-colored Di-I signals (A, B, Di-I stained cells; C, Control group; D-F, Experimental group. A, D are DIC images. B and E are fluorescence images).

• 

  Fig. 4 Photographs show sphincter muscle disruption and collagen deposit in the control group (A) at 4 weeks after injection. Sphincter muscles in the experimental group (B) were well preserved and intact, and with no collagen deposit (H&E, ×400).

• 

  Fig. 5 The immunohistochemical stainings for desmin and trichrome stain show apparent sphincter muscle disruption and collagen deposit in the control group (A&C, control group, B&D, experimental group, ×400).

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