J Korean Med Sci.  2007 Apr;22(2):198-204. 10.3346/jkms.2007.22.2.198.

Effect of Cosensitization with Buckwheat Flour Extract on the Production of House Dust Mite-specific IgE

• Shin YH
• Sohn MH
• Oh S
• Lee KE
• Yong TS
• Park JW
• Hong CS
• Kim KE
• Lee SY

• KMID: 1713160
• DOI: http://doi.org/10.3346/jkms.2007.22.2.198

Abstract

There are studies reporting food sensitization in infancy increases the risk of sensitization to inhalants later in life. We performed a study to evaluate whether cosensitization with buckwheat (BW) has an effect on the production of house dust mite-IgE. C3H/HeJ mice (4 weeks, female) were sensitized with house dust mite (HDM)/Al (OH)3, intraperitoneally on day 0, followed by 4 intranasal sensitizations (on days 14, 15, 16, and 21). Group 1 was cosensitized intragastrically with BW/cholera toxin (CT) (on days 0, 1, 2, 7, and 18) during sensitization with HDM, group 2 was cosensitized intragastrically with CT only (on days 0, 1, 2, 7, and 18), and group 3 was used as controls. HDM- and BW-IgE and antigen-specific T-cell proliferation and cytokine production were evaluated. In Group 1, BW-IgE levels were highest at week 4, and the HDM-IgE at week 3 (98.45+/-64.37 ng/mL and 169.86+/-55.54 ng/mL, respectively). In Group 2, HDM-IgE levels reached a peak at week 3, remarkably higher (810.52+/-233.29 ng/mL) compared to those of Group 1 (169.86+/-55.54 ng/mL). The interleukin (IL)-4 and interferon (IFN)-gamma in the HDM-stimulated culture supernatants of splenocytes were not significantly different among groups. We postulate that the cosensitization with BW may down-regulate the specific IgE response to HDM.

Keyword

Buckwheat; House Dust Mite; Immunoglobulin E; Bystander Effect; Animal Models

MeSH Terms

Pyroglyphidae/*immunology
Plant Extracts/adverse effects
Mice, Inbred C3H
Mice
Immunoglobulin E/*immunology
Immunization/*methods
Food Hypersensitivity/*immunology
Flour/*adverse effects
Female
Fagopyrum/*adverse effects
Disease Models, Animal
Dermatitis, Atopic/*immunology
Animals

Figure

• 

  Fig. 1 Sensitization and boost protocol for murine model of buckwheat and house dust mite allergy (each group, n=4). BW, buckwheat; CT, cholera toxin; HDM, house dust mite; S, sacrifice; i.p., intraperitoneal sensitization; i.n., intranasal sensitization.

• 

  Fig. 2 Serum levels of buckwheat-specific IgE (A) and house dust mite-specific IgE (B). Sera from different groups of mice (n=4) as indicated were obtained weekly after buckwheat/cholera toxin sensitization and/or house dust mite/alum sensitization. Buckwheat- and house dust mite-specific IgE levels in pooled sera from each group were determined by ELISA. Values are expressed as mean±SE (*, p<0.05 compared with group 3; †, p<0.05 compared with Group 1).

• 

  Fig. 3 Levels of IL-4 (A) and IFN-γ (B) in 72 hr spleen cell culture supernatant following stimulation with buckwheat, house dust mite, concanavalin A or media (n=4). Data are given as mean±SE.

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  Fig. 4 Serum levels of house dust mite-specific IgG1 (A) and IgG2a (B). Sera from different groups of mice (n=4) as indicated were obtained weekly after buckwheat/cholera toxin sensitization and/or house dust mite/alum sensitization. Buckwheat-specific IgG2a levels in pooled sera from each group were determined by ELISA. Values are expressed as mean±SE (*, p<0.05) (n=4).

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