Korean J Parasitol.
2013 Dec;51(6):637-644. 10.3347/kjp.2013.51.6.637.
Modulation of Antibody Responses against Gnathostoma spinigerum in Mice Immunized with Crude Antigen Formulated in CpG Oligonucleotide and Montanide ISA720
- Affiliations
-
- 1Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. pewpan@kku.ac.th
- 2Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
- 3Department of Microbiology, Faculty of Public Health, Mahidol University, Bangkok 10400, Thailand.
- 4Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
- KMID: 1712215
- DOI: http://doi.org/10.3347/kjp.2013.51.6.637
Abstract
- This study aimed to investigate the antibody responses in mice immunized with Gnathostoma spinigerum crude antigen (GsAg) incorporated with the combined adjuvant, a synthetic oligonucleotide containing unmethylated CpG motif (CpG ODN 1826) and a stable water in oil emulsion (Montanide ISA720). Mice immunized with GsAg and combined adjuvant produced all antibody classes and subclasses to GsAg except IgA. IgG2a/2b/3 but not IgG1 subclasses were enhanced by immunization with CpG ODN 1826 when compared with the control groups immunized with non-CpG ODN and Montanide ISA or only with Montanide ISA, suggesting a biased induction of a Th1-type response by CpG ODN. After challenge infection with live G. spinigerum larvae, the levels of IgG2a/2b/3 antibody subclasses decreased immediately and continuously, while the IgG1 subclass remained at high levels. This also corresponded to a continuous decrease of the IgG2a/IgG1 ratio after infection. Only IgM and IgG1 antibodies, but not IgG2a/2b/3, were significantly produced in adjuvant control groups after infection. These findings suggest that G. spinigerum infection potently induces a Th2-type biased response.
Keyword
MeSH Terms
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Adjuvants, Immunologic/*administration & dosage
Animals
Antibodies, Helminth/*blood
Antigens, Helminth/*administration & dosage/*immunology
Gnathostoma/*immunology
Immunoglobulin G/blood
Immunoglobulin M/blood
Male
Mannitol/administration & dosage/*analogs & derivatives
Mice
Oleic Acids/*administration & dosage
Oligodeoxyribonucleotides/*administration & dosage
Th1 Cells/immunology
Th2 Cells/immunology
Adjuvants, Immunologic
Antibodies, Helminth
Antigens, Helminth
Immunoglobulin G
Immunoglobulin M
Mannitol
Oleic Acids
Oligodeoxyribonucleotides
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