J Korean Acad Periodontol.  2005 Sep;35(3):799-811. 10.5051/jkape.2005.35.3.799.

The cytokine-inducing activities of surface components of the periodontopathogenic bacterium Porphyromonas gingivalis

• Kim SJ

• KMID: 1711778
• DOI: http://doi.org/10.5051/jkape.2005.35.3.799

Abstract

This study was carried out to examine the potency of the three surface components from Porphyromonas gingivalis to stimulate the murine macrophage cell line RAW264.7 to synthesize the pro-inflammatory cytokine tumor necrosis factor alpha(TNF-alpha) and nitric oxide (NO). Lipopolysaccharide(LPS), lipid A-associated proteins(LAP) and saline-extractable surface -associated material(SAM) were isolated from P. gingivalis 381. TNF-alpha release into culture supernatants was determined by two-site ELISA. NO production was assayed by measuring the accumulation of nitrite in culture supernatants. Western blot analysis of iNOS and analysis of reverse transcription(RT)-PCR products were carried out. The surface components extracted from this bacterium were almost equally potent in stimulating release of TNF-alpha and NO by RAW264.7 cells. TNF-alpha that was being measured immunologically was due to activation of TNF-alpha gene transcription. The present study clearly shows that P. gingivalis surface components fully induced iNOS expression in RAW264.7 cells in the absence of other stimuli. The ability of P. gingivalis surface components to promote the production of TNF-alpha and NO may be important in the pathogenesis of inflammatory periodontal disease.

Keyword

Porphyromonas gingivalis; Surface-associated material; Lipid A-associated proteins; Lipopoly-saccharide; TNF-alpha; Nitric oxide; iNOS

MeSH Terms

Tumor Necrosis Factor-alpha