J Vet Sci.  2013 Jun;14(2):227-230. 10.4142/jvs.2013.14.2.227.

Isolation and characterization of orf viruses from Korean black goats

• Oem JK
• Chung JY
• Kim YJ
• Lee KK
• Kim SH
• Jung BY
• Hyun BH

• KMID: 1705526
• DOI: http://doi.org/10.4142/jvs.2013.14.2.227

Abstract

Five cases of orf virus infection in Korean black goats were diagnosed in our laboratory between 2010 and 2011. One orf virus (ORF/2011) was isolated from an ovine testis cell line (OA3.Ts) for use as a vaccine candidate. Sequences of the major envelope protein and orf virus interferon resistance genes were determined and compared with published reference sequences. Phylogenetic analyses revealed that orf viruses from Korean black goats were most closely related to an isolate (ORF/09/Korea) from dairy goats in Korea. This result indicates that the orf viruses might have been introduced from dairy goats into the Korean black goat population.

Keyword

goat; isolation; orf virus; phylogenetic analysis

MeSH Terms

Animals
Ecthyma, Contagious/epidemiology/*virology
Goat Diseases/*epidemiology/virology
Goats
Molecular Sequence Data
Orf virus/*genetics/isolation & purification/metabolism
Phylogeny
Polymerase Chain Reaction/veterinary
Republic of Korea/epidemiology
Sequence Analysis, DNA/veterinary
Sequence Homology

Figure

• 

  Fig. 1 Phylogenetic analysis of different parapoxviruses based on nucleotide sequences of the B2L gene (A) and partial virus interferon resistance gene (B). Nucleotide sequences of different orf viruses were aligned using the Bioedit program and Mega 4 software. One thousand bootstrap replicates were used to determine nucleotide sequence distance. A consensus phylogenetic tree that was created using the neighbor-joining methods is shown.

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